ArticleScientific reports2019
Study of temporal variability of salivary cortisol and cortisone by LC-MS/MS using a new atmospheric pressure ionization source.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- Ashwagandha Root Extract Stabilises Physiological Stress Responses in Male and Female Team Sports Athletes During Pre-Season Training.Nutrients · 2026Trial
- Feasibility of home-based sampling of salivary cortisol and cortisone in healthy adults.BMC research notes · 2021Trial
- Sleep Architecture and Risk-Taking in Adolescents and Young Adults: Independent Contributions of REM Sleep, Sigma Activity, and Morning Cortisol.Behavioral sciences (Basel, Switzerland) · 2026Article
- Combination of magnetic bead extraction and LC-MS/MS detection for the detection of saliva cortisol.Analytical and bioanalytical chemistry · 2026Article
- Extraoral Detection of Biomarkers and Pathogens in Saliva: Comprehensive, Panoramic Review.Biosensors · 2026Review
- Article
- Non-invasive assessment of free steroid hormones: development of a high-throughput LC-MS/MS method for salivary steroid hormone quantification.Analytical and bioanalytical chemistry · 2025Article
- Awakening not associated with an increased rate of cortisol secretion.Proceedings. Biological sciences · 2025Article
- Mass Spectrometry-Based Metabolomics Reveals a Salivary Signature for Low-Severity COVID-19.International journal of molecular sciences · 2024Article
- Simultaneous Determination of Tobacco Smoke Exposure and Stress Biomarkers in Saliva Using In-Tube SPME and LC-MS/MS for the Analysis of the Association between Passive Smoking and Stress.Molecules (Basel, Switzerland) · 2024Article
- Plasma and serum metabolic analysis of healthy adults shows characteristic profiles by subjects' sex and age.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- Establishing Reference Intervals for Serum and Salivary Cortisol in a Selected Population in Kano, Nigeria.Annals of clinical and laboratory science · 2024Article
- Reliability of diurnal salivary cortisol metrics: A meta-analysis and investigation in two independent samples.Comprehensive psychoneuroendocrinology · 2023Article
- Confounding effects of liquorice, hydrocortisone, and blood contamination on salivary cortisol but not cortisone.Endocrine connections · 2023Article
- Molecularly Imprinted Polymer-Based Sensor for Electrochemical Detection of Cortisol.Biosensors · 2022Review
- Association of salivary steroid hormones and their ratios with time-domain heart rate variability indices in healthy individuals.Journal of medical biochemistry · 2021Article
Corrections and comments
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Authors and funding
5 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a growing interest concerning the relevance of salivary cortisone levels in stress-related research. However, studies investigating morning patterns and day-to-day variability of cortisone versus cortisol levels are lacking. Cortisol and cortisone analysis by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) has been widely used for routine laboratory measurements in the last years. The aim of this study was to develop an ultra-performance LC-MS/MS method for the simultaneous quantification of salivary cortisol and cortisone levels for assessing the temporal variability of these hormones. Saliva samples were collected from 18 healthy volunteers at 0, 15, and 30 min after awakening on each day for 1 week and analysed with the newly developed method. We used a novel atmospheric pressure ionization source, which resulted in high sensitivity and specificity for both cortisol and cortisone as well as higher peak values and signal-to-noise ratio as compared with the electrospray ionization source. Cortisone showed similar morning patterns as cortisol: a 25% and 49% increase in levels at 15 and 30 min after awakening, respectively. Most cortisone indices showed somewhat lower day-to-day variability and were less affected by state-related covariates. We recommend further exploration of the potential of salivary cortisone as a biomarker in stress-related research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.