ArticleScientific reports2019
Plant virome reconstruction and antiviral RNAi characterization by deep sequencing of small RNAs from dried leaves.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
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- Transcriptome and small RNAome profiling uncovers how a recombinant begomovirus evades RDRγ-mediated silencing of viral genes and outcompetes its parental virus in mixed infection.PLoS pathogens · 2024Article
- Cannabis Virome Reconstruction and Antiviral RNAi Characterization through Small RNA Sequencing.Plants (Basel, Switzerland) · 2023Article
- Divergent RNA viruses infecting sea lice, major ectoparasites of fish.PLoS pathogens · 2023Article
- Small RNA Profiling of Cucurbit Yellow Stunting Disorder Virus from Susceptible and Tolerant Squash (Viruses · 2023Article
- Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan.Pathogens (Basel, Switzerland) · 2023Article
- A Tale of Two Lobsters-Transcriptomic Analysis Reveals a Potential Gap in the RNA Interference Pathway in the Tropical Rock LobsterInternational journal of molecular sciences · 2022Article
- Plant Virome Analysis by the Deep Sequencing of Small RNAs of Fritillaria thunbergii var. chekiangensis and the Rapid Identification of Viruses.The plant pathology journal · 2022Article
- Comparative Plant Transcriptome Profiling of Arabidopsis thaliana Col-0 and Camelina sativa var.Microbiology spectrum · 2022Article
- Virus Elimination from Naturally Infected Field Cultivars of Potato (International journal of molecular sciences · 2022Article
- Contribution of historical herbarium small RNAs to the reconstruction of a cassava mosaic geminivirus evolutionary history.Scientific reports · 2021Article
- The Sisal Virome: Uncovering the Viral Diversity ofMicroorganisms · 2021Article
- Identification of a Novel Quinvirus in the FamilyFrontiers in microbiology · 2021Article
- Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1.BMC genomics · 2020Article
- Millet Could Be both a Weed and Serve as a Virus Reservoir in Crop Fields.Plants (Basel, Switzerland) · 2020Article
- Article
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4 authors.
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Abstract
In plants, RNA interference (RNAi) generates small interfering (si)RNAs from entire genomes of viruses, satellites and viroids. Therefore, deep small (s)RNA sequencing is a universal approach for virome reconstruction and RNAi characterization. We tested this approach on dried barley leaves from field surveys. Illumina sequencing of sRNAs from 2 plant samples identified in both plants Hordeum vulgare endornavirus (HvEV) and barley yellow mosaic bymovirus (BaYMV) and, additionally in one plant, a novel strain of Japanese soil-borne wheat mosaic furovirus (JSBWMV). De novo and reference-based sRNA assembly yielded complete or near-complete genomic RNAs of these viruses. While plant sRNAs showed broad size distribution, viral sRNAs were predominantly 21 and 22 nucleotides long with 5'-terminal uridine or adenine, and were derived from both genomic strands. These bona fide siRNAs are presumably processed from double-stranded RNA precursors by Dicer-like (DCL) 4 and DCL2, respectively, and associated with Argonaute 1 and 2 proteins. For BaYMV (but not HvEV, or JSBWMV), 24-nucleotide sRNAs represented the third most abundant class, suggesting DCL3 contribution to anti-bymovirus defence. Thus, viral siRNAs are well preserved in dried leaf tissues and not contaminated by non-RNAi degradation products, enabling both complete virome reconstruction and inference of RNAi components mediating antiviral defense.
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