Evidence map›Paper›PMID 31848353›Full record

ArticleScientific data2019

Profiling the effect of nafcillin on HA-MRSA D712 using bacteriological and physiological media.

Akanksha Rajput, Saugat Poudel, Hannah Tsunemoto, Michael Meehan, Richard Szubin, Connor A Olson, Anne Lamsa, Yara Seif, Nicholas Dillon, Alison Vrbanac and 9 more

Abstract readDataset
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific data, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Media Matters, Examining Historical and ModernFrontiers in cellular and infection microbiology · 2021
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Akanksha RajputDepartment of Bioengineering, University of California, San Diego, La Jolla, USA.
Saugat PoudelDepartment of Bioengineering, University of California, San Diego, La Jolla, USA.ORCID http://orcid.org/0000-0002-3732-2463
Hannah TsunemotoDivision of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.
Michael MeehanCollaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, USA.
Richard SzubinDepartment of Bioengineering, University of California, San Diego, La Jolla, USA.
Connor A OlsonDepartment of Bioengineering, University of California, San Diego, La Jolla, USA.
Anne LamsaDivision of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.
Yara SeifDepartment of Bioengineering, University of California, San Diego, La Jolla, USA.ORCID http://orcid.org/0000-0001-8813-5679
Nicholas DillonDepartment of Pediatrics, University of California, San Diego, La Jolla, CA, USA.
Alison VrbanacDepartment of Pediatrics, University of California, San Diego, La Jolla, CA, USA.
Joseph SugieDivision of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA.
Samira DaheshDepartment of Pediatrics, University of California, San Diego, La Jolla, CA, USA.
Jonathan M MonkDepartment of Bioengineering, University of California, San Diego, La Jolla, USA.
Pieter C DorresteinCollaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, California, USA.ORCID http://orcid.org/0000-0002-3003-1030
Rob KnightDepartment of Bioengineering, University of California, San Diego, La Jolla, USA.ORCID http://orcid.org/0000-0002-0975-9019
Victor NizetSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA.ORCID http://orcid.org/0000-0003-3847-0422
Bernhard O PalssonDepartment of Bioengineering, University of California, San Diego, La Jolla, USA.ORCID http://orcid.org/0000-0003-2357-6785
Adam M FeistDepartment of Bioengineering, University of California, San Diego, La Jolla, USA.
Joe PoglianoDivision of Biological Sciences, University of California San Diego, La Jolla, CA, 92093, USA. jpogliano@ucsd.edu.

Funding

Systems Biology Approach to Redefine Susceptibility Testing and Treatment of MDR Pathogens in the Context of Host ImmunityU01AI124316 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PALSSON, BERNHARD O · 2016 to 2020
$10.2M
NIAID NIH HHS U01 AI124316U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID) 1-U01-AI124316
6 · The paper itself

Abstract

Staphylococcus aureus strains have been continuously evolving resistance to numerous classes of antibiotics including methicillin, vancomycin, daptomycin and linezolid, compounding the enormous healthcare and economic burden of the pathogen. Cation-adjusted Mueller-Hinton broth (CA-MHB) is the standard bacteriological media for measuring antibiotic susceptibility in the clinical lab, but the use of media that more closely mimic the physiological state of the patient, e.g. mammalian tissue culture media, can in certain circumstances reveal antibiotic activities that may be more predictive of effectiveness in vivo. In the current study, we use both types of media to explore antibiotic resistance phenomena in hospital-acquired USA100 lineage methicillin-resistant, vancomycin-intermediate Staphylococcus aureus (MRSA/VISA) strain D712 via multidimensional high throughput analysis of growth rates, bacterial cytological profiling, RNA sequencing, and exo-metabolomics (HPLC and LC-MS). Here, we share data generated from these assays to shed light on the antibiotic resistance behavior of MRSA/VISA D712 in both bacteriological and physiological media.

Indexed as

Drug Resistance, Multiple, BacterialCulture MediaHigh-Throughput Screening AssaysMetabolomicsMethicillin-Resistant Staphylococcus aureusNafcillinSequence Analysis, RNACulture MediaNafcillin

Identifiers

PMID31848353
PMCPMC6917727

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.