ArticleMolecular and cellular probes2020
Epidemiological investigation of feline infectious peritonitis in cats living in Harbin, Northeast China from 2017 to 2019 using a combination of an EvaGreen-based real-time RT-PCR and serum chemistry assays.
Article in Molecular and cellular probes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Double-Antibody Sandwich Enzyme-Linked Immunosorbent Assay for Detecting Feline Coronavirus Infection.Veterinary medicine and science · 2026Article
- The use of high-resolution melting analysis to distinguish feline enteric and feline infectious peritonitis coronaviruses.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Validation and comparison of EvaGreen- and TaqMan-based real-time qRT-PCR for diagnosis of the black queen cell virus in the honey bees.Veterinary research communications · 2025Article
- A review of feline infectious peritonitis virus infection.Veterinary world · 2024Review
- Prevalence and Risk Factors Associated with Feline Infectious Peritonitis (FIP) in Mainland China between 2008 and 2023: A Systematic Review and Meta-Analysis.Animals : an open access journal from MDPI · 2024Article
- Prevalence of natural feline coronavirus infection in domestic cats in Fujian, China.Virology journal · 2024Article
- Development of multiplex real-time PCR for simultaneous detection of SARS-CoV-2, CCoV, and FIPV.Frontiers in veterinary science · 2024Article
- Genetic Diversity and Evolution of Viruses InfectingViruses · 2023Review
- Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus.Viruses · 2021Article
- A survey of jaagsiekte sheep retrovirus (JSRV) infection in sheep in the three northeastern provinces of China.Archives of virology · 2021Article
- Lectin Protein as a Promising Component to Functionalize Micelles, Liposomes and Lipid NPs against Coronavirus.Biomedicines · 2020Review
- Prevalence of Feline Coronavirus Shedding in German Catteries and Associated Risk Factors.Viruses · 2020Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Feline infectious peritonitis (FIP) is caused by the FIP virus (FIPV), a highly virulent mutant form of feline coronavirus (FCoV). This disease is one of the most important infectious diseases in cats, and it is associated with high mortality, particularly among younger cats. In this study, we isolated a wild-type FIPV HRB-17 epidemic strain from the blood sample of household pet cat exhibiting the characteristic wet-form FIP symptoms, which has been confirmed further by animal infection. Further, we developed an EvaGreen-based real-time RT-PCR assay for the accurate detection of FCoV based on the amplification of the highly conserved FIPV N gene. Then, using a combination of the real-time RT-PCR approach and a serum chemistry assay, we performed an epidemiological survey of FIPV infection in cats living in Harbin City, Northeast China. The results indicated that the EvaGreen-based real-time RT-PCR assay can be used for screening FCoV infection in the affected cats at an analytical detection limit of 8.2 × 10
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