ArticlePlant biotechnology journal2020
Genetic modulation of RAP alters fruit coloration in both wild and cultivated strawberry.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 103 citations in OpenAlex.
- Multifaceted mechanisms underlying species- and cultivar-associated differential anthocyanin accumulation in fruits.Horticulture research · 2026Article
- Boosting genome editing in perennial plants by CRISPR-Combo mediated morphogenic gene activation.Nature communications · 2026Article
- AaMYB114 promotes the coloration of Actinidia arguta by synthesizing and transporting anthocyanins.BMC plant biology · 2026Article
- The telomere-to-telomere haplotype genome provides in-depth insights into the molecular mechanisms of the anthocyanin deficiency phenotype in Prunus mume.Molecular horticulture · 2026Article
- Genome-wide analysis of leucine-rich repeat-only proteins gene family revealed FaLRRop26 responding Colletotrichum Siamense infection by regulated lignin synthesis in cultivated strawberry (Fragaria × ananassa).BMC plant biology · 2026Article
- Whitening fruit by CRISPR/Cas9-mediated homoeolog-specific gene editing ofHorticulture research · 2026Article
- Strawberry cryptochrome FvCRY1 and FvCRY2 transcriptionally regulate anthocyanin biosynthesis and sugar metabolism.Molecular horticulture · 2025Article
- Plant synthetic biology-based biofortification, strategies and recent progresses.Journal of integrative plant biology · 2025Review
- Overexpression of housekeeping geneHorticulture research · 2025Article
- Recent advances in biosynthesis and regulation of strawberry anthocyanins.Horticulture research · 2025Article
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- Spatial metabolic heterogeneity of sugar-acid balance and pigment accumulation in distinct color regions ofFrontiers in plant science · 2025Article
- Enhancing quality and climate resilient traits in vegetatively propagated polyploids: transgenic and genome editing advancements, challenges and future directions.Frontiers in genetics · 2025Review
- Genome editing for sustainable agriculture in Peru: advances, potential applications and regulation.Frontiers in genome editing · 2025Review
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- Developing Iron and Iodine Enrichment in Tomato Fruits to Meet Human Nutritional Needs.Plants (Basel, Switzerland) · 2024Review
- Loss-of-function mutation in anthocyanidin reductase activates the anthocyanin synthesis pathway in strawberry.Molecular horticulture · 2024Article
- Downregulation of a Phi class glutathionePlant biotechnology (Tokyo, Japan) · 2024Article
- Combined Widely Targeted Metabolomic, Transcriptomic, and Spatial Metabolomic Analysis Reveals the Potential Mechanism of Coloration and Fruit Quality Formation inFoods (Basel, Switzerland) · 2024Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fruit colour affects consumer preference and is an important trait for breeding in strawberry. Previously, we isolated the Reduced Anthocyanins in Petioles (RAP) gene encoding a glutathione S-transferase (GST) that binds anthocyanins to facilitate their transport from cytosol to vacuole in the diploid strawberry Fragaria vesca. The parent of rap was the F. vesca variety 'Yellow Wonder' that develops white fruit due to a natural mutation in the FveMYB10 gene. Here, we investigated the application potential of RAP in modulating fruit colours by overexpression of RAP in F. vesca and knockout of RAP in the cultivated strawberry Fragaria × ananassa. Unexpectedly, the RAP overexpression in Yellow Wonder background caused formation of red fruit. In addition, the red coloration occurs precociously at floral stage 10 and continues in the receptacle during early fruit development. Transcriptome analysis revealed that the anthocyanin biosynthesis genes were not up-regulated in RAP-ox; rap myb10 flowers at anthesis and largely inhibited at the turning stage in fruit, suggesting a coloration mechanism independent of FveMYB10. Moreover, we used CRISPR/Cas9 to knockout RAP in cultivated strawberry which is octoploid. Six copies of RAP were simultaneously knocked out in the T0 generation leading to the green stem and white-fruited phenotype. Several T1 progeny have segregated away the CRISPR/Cas9 transgene but maintain the green stem trait. Our results indicate that enhancing the anthocyanin transport could redirect the metabolic flux from proanthocyanidin to anthocyanin production at early developmental stages of fruit and that RAP is one promising candidate gene in fruit colour breeding of strawberry.
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