Evidence map›Paper›PMID 31844934›Full record

ArticleCurrent microbiology2020

In Vitro Cytotoxicity of Parasporins from Native Algerian Bacillus thuringiensis Strains Against Laryngeal and Alveolar Cancers.

Lila Aberkane, Assia Nacer-Khodja, Zahia Djenane, Lydia Neila Djouadi, Abdelhakim Ouafek, Lamjed Bouslama, Hocine Grib, Nabil Mameri, Farida Nateche, Assia Djefal

Abstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. AX-2: A Promising Non-Hemolytic Protein ofMolecules (Basel, Switzerland) · 2026
    Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Lila AberkaneMedical Applications Department, Nuclear Applications Division, Nuclear Research Center of Algiers (CRNA), 02 Bd Frantz Fanon, P.O. Box 399, 16000, Algiers-Gare, Algiers, Algeria.
Assia Nacer-KhodjaMedical Applications Department, Nuclear Applications Division, Nuclear Research Center of Algiers (CRNA), 02 Bd Frantz Fanon, P.O. Box 399, 16000, Algiers-Gare, Algiers, Algeria.
Zahia DjenaneLaboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), P.O. Box 32, El Alia, 16111, Bab Ezzouar, Algiers, Algeria.
Lydia Neila DjouadiLaboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), P.O. Box 32, El Alia, 16111, Bab Ezzouar, Algiers, Algeria.
Abdelhakim OuafekMedical Applications Department, Nuclear Applications Division, Nuclear Research Center of Algiers (CRNA), 02 Bd Frantz Fanon, P.O. Box 399, 16000, Algiers-Gare, Algiers, Algeria.
Lamjed BouslamaLaboratory of Bioactive Substances, Center of Biotechnology of Borj Cedria (CBBC), University of Tunis El Manar, P.O. Box 901, 2050, Hammam-Lif, Tunisia.
Hocine GribEnvironmental Engineering Department, National Polytechnic School of Algiers (ENP), 10 Rue des Frères Oudek, Hacène Badi, PO Box 182, 16200, El Harrach, Algiers, Algeria.
Nabil MameriEnvironmental Engineering Department, National Polytechnic School of Algiers (ENP), 10 Rue des Frères Oudek, Hacène Badi, PO Box 182, 16200, El Harrach, Algiers, Algeria.
Farida NatecheLaboratory of Cellular and Molecular Biology (LCMB), Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), P.O. Box 32, El Alia, 16111, Bab Ezzouar, Algiers, Algeria.
Assia DjefalMedical Applications Department, Nuclear Applications Division, Nuclear Research Center of Algiers (CRNA), 02 Bd Frantz Fanon, P.O. Box 399, 16000, Algiers-Gare, Algiers, Algeria. a.djefal@crna.dz.
Centre de Recherche Nucléaire d’Alger · DZUniversity of Sciences and Technology Houari Boumediene · DZPolytechnic School of Algiers · DZCenter of Biotechnogy of Borj Cédria · TN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Parasporins (PS), a class of non-insecticidal and non-hemolytic crystal proteins of Bacillus thuringiensis (Bt), are being explored as promising anti-cancer agents due to their specific toxicity to cancer cells. This work is considered as a first initiative aiming at investigating Algerian soil Bt isolates' activity and cytotoxic potential against cancer cells. A total of 48 Bacillus spp. were isolated from different sites in Algeria. Phenotypic and biochemical tests, 16S rDNA molecular identification, and microscopic observation of crystal have confirmed the identification of Bt for ten strains. A screening for non-hemolytic crystalline proteins was performed. Extraction, purification, and activation of non-hemolytic proteins by chromatographic analysis yielded several polypeptides of different molecular weights. A purified PS1, with pro-protein of 81 kDa and several peptides with different molecular weights (18-58 kDa) after activation by trypsin, has been identified from the strain BDzG. The NH

Indexed as

A549 CellsAlgeriaAntineoplastic AgentsBacillus thuringiensisCell Line, TumorCell SurvivalEndotoxinsEpithelial CellsHumansInhibitory Concentration 50Laryngeal NeoplasmsLung NeoplasmsSoil MicrobiologyAntineoplastic AgentsEndotoxinsparasporin

Identifiers

PMID31844934
OpenAlexW2995126861

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.