ArticleScience advances2019
Focusing light inside live tissue using reversibly switchable bacterial phytochrome as a genetically encoded photochromic guide star.
Article in Science advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- Near-infrared photochromic miniprotein enables photoacoustic molecular imaging of acute inflammation and intracellular antigens.Nature methods · 2026Article
- Focusing scattered light through live tissue with channel-selected wavefront shaping.Biomedical optics express · 2026Article
- Advances in Photonic Materials and Integrated Devices for Smart and Digital Healthcare: Bridging the Gap Between Materials and Systems.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Optical focusing into scattering media via iterative time reversal guided by absorption nonlinearity.Nature communications · 2025Article
- Recent advancements in molecular photoacoustic tomography.JPhys photonics · 2025Review
- Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout.Nature communications · 2025Article
- Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation.Nature communications · 2024Article
- Reversibly photoswitchable protein assemblies with collagen affinity for in vivo photoacoustic imaging of tumors.Science advances · 2024Article
- Challenges and advances in two-dimensional photoacoustic computed tomography: a review.Journal of biomedical optics · 2024Review
- Multiple photofluorochromic luminogens via catalyst-free alkene oxidative cleavage photoreaction for dynamic 4D codes encryption.Nature communications · 2024Article
- Wavefront shaping improves the transparency of the scattering media: a review.Journal of biomedical optics · 2024Review
- Toward Tunable Protein-Driven Hydrogel Lens.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Optophysiology: Illuminating cell physiology with optogenetics.Physiological reviews · 2022Review
- Transgenic mice encoding modern imaging probes: Properties and applications.Cell reports · 2022Review
- Enhance the delivery of light energy ultra-deep into turbid medium by controlling multiple scattering photons to travel in open channels.Light, science & applications · 2022Article
- Background-suppressed tumor-targeted photoacoustic imaging using bacterial carriers.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- Focusing light into scattering media with ultrasound-induced field perturbation.Light, science & applications · 2021Article
- High-speed photoacoustic-guided wavefront shaping for focusing light in scattering media.Optics letters · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Focusing light deep by engineering wavefronts toward guide stars inside scattering media has potential biomedical applications in imaging, manipulation, stimulation, and therapy. However, the lack of endogenous guide stars in biological tissue hinders its translations to in vivo applications. Here, we use a reversibly switchable bacterial phytochrome protein as a genetically encoded photochromic guide star (GePGS) in living tissue to tag photons at targeted locations, achieving light focusing inside the tissue by wavefront shaping. As bacterial phytochrome-based GePGS absorbs light differently upon far-red and near-infrared illumination, a large dynamic absorption contrast can be created to tag photons inside tissue. By modulating the GePGS at a distinctive frequency, we suppressed the competition between GePGS and tissue motions and formed tight foci inside mouse tumors in vivo and acute mouse brain tissue, thus improving light delivery efficiency and specificity. Spectral multiplexing of GePGS proteins with different colors is an attractive possibility.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.