Evidence map›Paper›PMID 31844155›Full record

ArticleNature biomedical engineering2020

Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation.

Zhaogang Yang, Junfeng Shi, Jing Xie, Yifan Wang, Jingyao Sun, Tongzheng Liu, Yarong Zhao, Xiuting Zhao, Xinmei Wang, Yifan Ma and 19 more

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biomedical engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 412 papers.

0numbers the graph read from it
0cells of the map it votes in
412citing papers in PubMed
24.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

412 citing papers in PubMed, 700 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Review
  14. Review
  15. Review
  16. Article
  17. AI-nanotech synergies: recent advances in sustainable bio-manufacturing.World journal of microbiology & biotechnology · 2026
    Review
  18. Anti-CD19-engineered exosomes enable B cell-targeted anti-BAFF mRNA delivery to alleviate lupus progression.Molecular therapy : the journal of the American Society of Gene Therapy · 2026
    Article
  19. Review
  20. Review

352 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

29 authors at 11 institutions in 2 countries.

Zhaogang Yang *Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Junfeng Shi *Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Jing XieSchool of Life Sciences, Jilin University, Changchun, China.
Yifan WangDepartment of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX, USA.ORCID http://orcid.org/0000-0001-7387-0654
Jingyao SunDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Tongzheng LiuCollege of Pharmacy, Jinan University, Guangzhou, China.
Yarong ZhaoSchool of Life Sciences, Jilin University, Changchun, China.
Xiuting ZhaoSchool of Life Sciences, Jilin University, Changchun, China.
Xinmei WangDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Yifan MaDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Veysi MalkocDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Chiling ChiangCollege of Medicine, The Ohio State University, Columbus, OH, USA.
Weiye DengDepartment of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX, USA.ORCID http://orcid.org/0000-0003-4243-5013
Yuanxin ChenDepartment of Neurological Surgery, Mayo Clinic, Jacksonville, FL, USA.
Yuan FuDepartment of Neurological Surgery, Mayo Clinic, Jacksonville, FL, USA.
Kwang J KwakDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Yamin FanDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Chen KangCarver College of Medicine, University of Iowa, Iowa City, IA, USA.
Changcheng YinDepartment of Biophysics, Health Science Center, Peking University, Beijing, China.
June RheeInstitute for Stem Cell Biology, Stanford University, Palo Alto, CA, USA.
Paul BertaniDepartment of Electrical and Computer Engineering, The Ohio State University, Columbus, OH, USA.
Jose OteroDepartment of Neuroscience, The Ohio State University, Columbus, OH, USA.
Wu LuDepartment of Electrical and Computer Engineering, The Ohio State University, Columbus, OH, USA.
Kyuson YunPeak Center for Pituitary and Brain Tumor, Department of Neurosurgery, Houston Methodist Research institute, Weill-Cornell Medical College, Houston, TX, USA.
Andrew S LeeInstitute for Stem Cell Biology, Stanford University, Palo Alto, CA, USA.
Wen JiangDepartment of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX, USA.ORCID http://orcid.org/0000-0001-9154-633X
Lesheng TengSchool of Life Sciences, Jilin University, Changchun, China. tenglesheng@jlu.edu.cn.ORCID http://orcid.org/0000-0003-1623-5384
Betty Y S KimDepartment of Neurological Surgery, Mayo Clinic, Jacksonville, FL, USA. bykim@mdanderson.org.ORCID http://orcid.org/0000-0001-6890-8355
L James LeeDepartment of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA. lee.31@osu.edu.ORCID http://orcid.org/0000-0002-3589-0280
The Ohio State University · USJilin University · CNThe University of Texas Southwestern Medical Center · USWinnMed · USHouston Methodist · USJinan University · CNPeking University · CNShenzhen Bay Laboratory · CNStanford University · USThe University of Texas MD Anderson Cancer Center · USUniversity of Iowa · US

Funding

Tumor Evolution and Metastasis ProgramP30CA016672 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI Scott Kopetz · 1985 to 2026
$290.8M
Mechanisms of Congenital HypoventilationR01HL132355 · NHLBI · OHIO STATE UNIVERSITY · PI CZEISLER, CATHERINE, OTERO, JOSE JAVIER · 2016 to 2020
$2.5M
Therapeutic modulation of the phagocytosis axis as a novel glioblastoma immunotherapyR01NS104315 · NINDS · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI KIM, BETTY · 2018 to 2022
$1.7M
Microfluidics Array Based Sorting, Isolation, and RNA Analysis in Single Extracellular V csiclesUG3TR002884 · NCATS · OHIO STATE UNIVERSITY · PI KIM, BETTY, REATEGUI, EDUARDO · 2019 to 2020
$822k
A Phagocytosis Modulating Nanomedicine for Targeted Breast Cancer ImmunotherapyK08CA241070 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI JIANG, WEN · 2019 to 2021
$760k
Transmission Electron MicroscopeS10RR026780 · NCRR · STANFORD UNIVERSITY · PI TALBOT, WILLIAM S · 2010 to 2010
$412k
NCATS NIH HHS UG3 TR002884NCI NIH HHS K08 CA241070NCI NIH HHS P30 CA016672NCRR NIH HHS S10 RR026780NHLBI NIH HHS R01 HL132355NINDS NIH HHS R01 NS104315
6 · The paper itself

Abstract

Exosomes are attractive as nucleic-acid carriers because of their favourable pharmacokinetic and immunological properties and their ability to penetrate physiological barriers that are impermeable to synthetic drug-delivery vehicles. However, inserting exogenous nucleic acids, especially large messenger RNAs, into cell-secreted exosomes leads to low yields. Here we report a cellular-nanoporation method for the production of large quantities of exosomes containing therapeutic mRNAs and targeting peptides. We transfected various source cells with plasmid DNAs and stimulated the cells with a focal and transient electrical stimulus that promotes the release of exosomes carrying transcribed mRNAs and targeting peptides. Compared with bulk electroporation and other exosome-production strategies, cellular nanoporation produced up to 50-fold more exosomes and a more than 10

Indexed as

Drug Delivery SystemsAnimalsBrain NeoplasmsCells, CulturedDisease Models, AnimalElectroporationExosomesGliomaHEK293 CellsHumansMice, Inbred C57BLMicroRNAsNanotechnologyRNA, MessengerSignal TransductionMicroRNAsRNA, Messenger

Identifiers

PMID31844155
PMCPMC7080209
OpenAlexW2995128645

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.