ArticleScientific reports2019
Protein components of ribonucleoprotein granules from Drosophila germ cells oligomerize and show distinct spatial organization during germline development.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Germ granule-mediated mRNA storage and translational control.RNA biology · 2025Review
- Multiple domains of scaffold Tudor protein play nonredundant roles inLife science alliance · 2025Article
- Review
- Diverse roles of stress-responsive RNP granules in oogenesis and infertility.Biology of reproduction · 2025Review
- Novel structure and composition of the unusually large germline determinant of the wasp Nasonia vitripennis.Molecular biology of the cell · 2025Article
- The piRNA protein Asz1 is essential for germ cell and gonad development in zebrafish and exhibits differential necessities in distinct types of germ granules.PLoS genetics · 2025Article
- Dynamic protein assembly and architecture of the large solitary membraneless organelle during germline development in the wasp Nasonia vitripennis.Development (Cambridge, England) · 2024Article
- How germ granules promote germ cell fate.Nature reviews. Genetics · 2024Review
- Novel structure and composition of the unusually large germline determinant of the wasp Nasonia vitripennis.bioRxiv : the preprint server for biology · 2024Article
- Spatial organization of translation and translational repression in two phases of germ granules.Nature communications · 2024Article
- Me31B: a key repressor in germline regulation and beyond.Bioscience reports · 2024Review
- Plasticity of Drosophila germ granules during germ cell development.PLoS biology · 2023Article
- Germ granules in development.Development (Cambridge, England) · 2023Article
- Specialized germline P-bodies are required to specify germ cell fate in Caenorhabditis elegans embryos.Development (Cambridge, England) · 2022Article
- Computational modeling offers new insight into Drosophila germ granule development.Biophysical journal · 2022Article
- A Single-Cell Omics Network Model of Cell Crosstalk during the Formation of Primordial Follicles.Cells · 2022Article
- It's Just a Phase: Exploring the Relationship Between mRNA, Biomolecular Condensates, and Translational Control.Frontiers in genetics · 2022Review
- Looking at the Pretty "Phase" of Membraneless Organelles: A View FromFrontiers in cell and developmental biology · 2022Review
- Modulation of Aub-TDRD interactions elucidates piRNA amplification and germplasm formation.Life science alliance · 2021Article
- Glial granules contain germline proteins in the Drosophila brain, which regulate brain transcriptome.Communications biology · 2020Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assembly of large RNA-protein granules occurs in germ cells of many animals and these germ granules have provided a paradigm to study structure-functional aspects of similar structures in different cells. Germ granules in Drosophila oocyte's posterior pole (polar granules) are composed of RNA, in the form of homotypic clusters, and proteins required for germline development. In the granules, Piwi protein Aubergine binds to a scaffold protein Tudor, which contains 11 Tudor domains. Using a super-resolution microscopy, we show that surprisingly, Aubergine and Tudor form distinct clusters within the same polar granules in early Drosophila embryos. These clusters partially overlap and, after germ cells form, they transition into spherical granules with the structural organization unexpected from these interacting proteins: Aubergine shell around the Tudor core. Consistent with the formation of distinct clusters, we show that Aubergine forms homo-oligomers and using all purified Tudor domains, we demonstrate that multiple domains, distributed along the entire Tudor structure, interact with Aubergine. Our data suggest that in polar granules, Aubergine and Tudor are assembled into distinct phases, partially mixed at their "interaction hubs", and that association of distinct protein clusters may be an evolutionarily conserved mechanism for the assembly of germ granules.
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