ArticleBiophysical journal2020
Weak Acid Permeation in Synthetic Lipid Vesicles and Across the Yeast Plasma Membrane.
Article in Biophysical journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
- Functional In Vitro Expression of the Succinate-Acetate Transporter SatP and an Initial Screening Strategy.Membranes · 2026Article
- Protonophore activity of short-chain fatty acids induces their intracellular accumulation and acidification.FEBS letters · 2025Article
- Impact of GRAS compounds applied by ultrasonic nebulization on decay control and quality parameters in Persian lime.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Deriving Membrane-Water and Protein-Water Partition Coefficients from In Vitro Experiments for Per- and Polyfluoroalkyl Substances (PFAS).Environmental science & technology · 2025Article
- Response mechanism of Saccharomyces cerevisiae under benzoic acid stress in ethanol fermentation.Scientific reports · 2024Article
- Formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficient Komagataella phaffii.Microbial biotechnology · 2024Article
- Improving the Accuracy of Permeability Data to Gain Predictive Power: Assessing Sources of Variability in Assays Using Cell Monolayers.Membranes · 2024Review
- The role of ion homeostasis in adaptation and tolerance to acetic acid stress in yeasts.FEMS yeast research · 2024Review
- Prodigiosin-Functionalized Probiotic Ghosts as a Bioinspired Combination Against Colorectal Cancer Cells.Probiotics and antimicrobial proteins · 2023Article
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- Weak acids produced during anaerobic respiration suppress both photosynthesis and aerobic respiration.Nature communications · 2023Article
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- A Hitchhiker's Guide to Supplying Enzymatic Reducing Power into Synthetic Cells.ACS synthetic biology · 2023Review
- Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective.ACS synthetic biology · 2023Review
- Determining small-molecule permeation through lipid membranes.Nature protocols · 2022Review
- Quantifying proton-induced membrane polarization in single biomimetic giant vesicles.Biophysical journal · 2022Article
- Membrane thickness, lipid phase and sterol type are determining factors in the permeability of membranes to small solutes.Nature communications · 2022Article
- coupled Hydrodynamic Flow Focusing (cHFF) to Engineer Lipid-Polymer Nanoparticles (LiPoNs) for Multimodal Imaging and Theranostic Applications.Biomedicines · 2022Article
- Loss of the Acetate Switch in Vibrio vulnificus Enhances Predation Defense against Tetrahymena pyriformis.Applied and environmental microbiology · 2022Article
- Lactic Acid Permeability of Aquaporin-9 Enables Cytoplasmic Lactate Accumulation via an Ion Trap.Life (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a fluorescence-based approach for determination of the permeability of small molecules across the membranes of lipid vesicles and living cells. With properly designed experiments, the method allows us to assess the membrane physical properties both in vitro and in vivo. We find that the permeability of weak acids increases in the order of benzoic > acetic > formic > lactic, both in synthetic lipid vesicles and the plasma membrane of Saccharomyces cerevisiae, but the permeability is much lower in yeast (one to two orders of magnitude). We observe a relation between the molecule permeability and the saturation of the lipid acyl chain (i.e., lipid packing) in the synthetic lipid vesicles. By analyzing wild-type yeast and a manifold knockout strain lacking all putative lactic acid transporters, we conclude that the yeast plasma membrane is impermeable to lactic acid on timescales up to ∼2.5 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.