Evidence map›Paper›PMID 31842978›Full record

ArticleStem cell research & therapy2019

Mesenchymal stem cell-derived exosomal microRNA-133b suppresses glioma progression via Wnt/β-catenin signaling pathway by targeting EZH2.

Haiyang Xu, Guifang Zhao, Yu Zhang, Hong Jiang, Weiyao Wang, Donghai Zhao, Jin Hong, Hongquan Yu, Ling Qi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
105citing papers in PubMed, 2 pooled it
6.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

105 citing papers in PubMed, 2 syntheses or guidelines pooled it, 157 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Article
  6. Review
  7. Review
  8. Review
  9. Review
  10. Article
  11. Article
  12. Review
  13. Review
  14. Review
  15. Review
  16. Review
  17. Review
  18. Review
  19. Review
  20. Review

45 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Haiyang XuDepartment of Oncological Neurosurgery, First Hospital of Jilin University, No. 71, Xinmin Street, Changchun, 130021, Jilin Province, People's Republic of China.
Guifang ZhaoThe Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, B24, Yinquan South Road, Qingyuan, 511518, Guangdong Province, People's Republic of China.
Yu ZhangDepartment of Neurovascular, First Hospital of Jilin University, Changchun, 130021, People's Republic of China.
Hong JiangDepartment of Ophthalmology, China-Japan Union Hospital of Jilin University, Changchun, 130033, People's Republic of China.
Weiyao WangDepartment of Pathophysiology, Jilin Medical University, No. 5, Jilin Street, Jilin, 132013, Jilin Province, People's Republic of China.
Donghai ZhaoDepartment of Pathophysiology, Jilin Medical University, No. 5, Jilin Street, Jilin, 132013, Jilin Province, People's Republic of China.
Jin HongDepartment of Pathophysiology, Jilin Medical University, No. 5, Jilin Street, Jilin, 132013, Jilin Province, People's Republic of China.
Hongquan YuDepartment of Oncological Neurosurgery, First Hospital of Jilin University, No. 71, Xinmin Street, Changchun, 130021, Jilin Province, People's Republic of China. anglitangjing@126.com.
Ling QiThe Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, B24, Yinquan South Road, Qingyuan, 511518, Guangdong Province, People's Republic of China. qilingip@126.com.
Jilin Medical University · CNFirst Hospital of Jilin University · CNGuangzhou Medical University · CNUnion Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMesenchymal stem cells (MSCs) play a significant role in cancer initiation and metastasis, sometimes by releasing exosomes that mediate cell communication by delivering microRNAs (miRNAs). This study aimed to investigate the effects of exosomal miR-133b derived from MSCs on glioma cell behaviors.

methodsMicroarray-based analysis identified the differentially expressed genes (DEGs) in glioma. The expression patterns of EZH2 and miR-133b along with interaction between them were clarified in glioma. The expression of miR-133b and EZH2 in glioma cells was altered to examine their functions on cell activities. Furthermore, glioma cells were co-cultured with MSC-derived exosomes treated with miR-133b mimic or inhibitor, and EZH2-over-expressing vectors or shRNA against EZH2 to characterize their effect on proliferation, invasion, and migration of glioma cells in vitro. In vivo assays were also performed to validate the in vitro findings.

resultsmiR-133b was downregulated while EZH2 was upregulated in glioma tissues and cells. miR-133b was found to target and negatively regulate EZH2 expression. Moreover, EZH2 silencing resulted in inhibited glioma cell proliferation, invasion, and migration. Additionally, MSC-derived exosomes containing miR-133b repressed glioma cell proliferation, invasion, and migration by inhibiting EZH2 and the Wnt/β-catenin signaling pathway. Furthermore, in vivo experiments confirmed the tumor-suppressive effects of MSC-derived exosomal miR-133b on glioma development.

conclusionCollectively, the obtained results suggested that MSC-derived exosomes carrying miR-133b could attenuate glioma development via disrupting the Wnt/β-catenin signaling pathway by inhibiting EZH2, which provides a potential treatment biomarker for glioma.

Indexed as

Wnt Signaling PathwayAnimalsBrain NeoplasmsCell Line, TumorCell MovementCell ProliferationCoculture TechniquesDisease ProgressionEnhancer of Zeste Homolog 2 ProteinExosomesFemaleGene Expression Regulation, NeoplasticGliomaHumansMesenchymal Stem CellsMiceEnhancer of Zeste Homolog 2 ProteinEZH2 protein, humanMicroRNAsMIRN133 microRNA, humanExosomesEZH2GliomaMesenchymal stem cellsMicroRNA-133bWnt/β-catenin signaling pathway

Identifiers

PMID31842978
PMCPMC6915914
OpenAlexW2995274886

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.