ArticleBMC urology2019
Quantitative proteomic characterization of human sperm cryopreservation: using data-independent acquisition mass spectrometry.
Article in BMC urology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Proteomic Profiling of Cryopreservation-Induced Alterations in Rhesus Macaque Sperm.Andrology · 2026Article
- Metabolic pathways fueling human spermatozoa capacitation and hyperactivation: a roadmap for spermatozoa bioenergetics.Frontiers in endocrinology · 2026Review
- Reactive oxygen species and antioxidant strategies in human sperm cryodamage.Frontiers in medicine · 2026Review
- Semen cryopreservation and storage in liquid nitrogen: Impact on chromatin compaction.Andrology · 2025Article
- Engineered extracellular vesicles: a breakthrough approach to overcoming sperm cryopreservation challenges.Reproductive biology and endocrinology : RB&E · 2025Review
- Combined Analysis of the Transcriptome, Proteome and Metabolome in Human Cryopreserved Sperm.The world journal of men's health · 2024Article
- Enhancement of Frozen-Thawed Human Sperm Quality with Zinc as a Cryoprotective Additive.Medical science monitor : international medical journal of experimental and clinical research · 2024Article
- Proteomics techniques in protein biomarker discovery.Quantitative biology (Beijing, China) · 2024Review
- Long-term storage modifies the microRNA expression profile of cryopreserved human semen.Biomolecules & biomedicine · 2024Article
- Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm-Oocyte Interaction.Journal of proteome research · 2023Article
- Integrative Bioinformatics Analysis of The Cell Division Cycle and Ribosomal Pathways in The Rat Varicocele: Implications for Drug Discovery.Cell journal · 2023Article
- Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects.International journal of molecular sciences · 2023Review
- Dietary flaxseed oil and vitamin E improve semen qualityFrontiers in endocrinology · 2023Article
- Article
- Integrated multi-omics analyses reveals molecules governing sperm metabolism potentially influence bull fertility.Scientific reports · 2022Article
- Sperm Phosphoproteome: Unraveling Male Infertility.Biology · 2022Review
- Proteomics and Co-expression Network Analysis Reveal the Importance of Hub Proteins and Metabolic Pathways in Nicotine Synthesis and Accumulation in Tobacco (Frontiers in plant science · 2022Article
- The cryopreservation process induces alterations in proteins associated with bull sperm quality: The equilibration process could be a probable critical control point.Frontiers in endocrinology · 2022Article
- Quantitative phosphoproteomics reveals GSK3A substrate network is involved in the cryodamage of sperm motility.Bioscience reports · 2021Article
- In Stallion Spermatozoa, Superoxide Dismutase (Cu-Zn) (SOD1) and the Aldo-Keto-Reductase Family 1 Member b (AKR1B1) Are the Proteins Most Significantly Reduced by Cryopreservation.Journal of proteome research · 2021Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
Abstract
backgroundHuman sperm cryopreservation is a simple and effective approach for male fertility preservation.
methodsTo identify potential proteomic changes in this process, data-independent acquisition (DIA), a technology with high quantitative accuracy and highly reproducible proteomics, was used to quantitatively characterize the proteomics of human sperm cryopreservation.
resultsA total of 174 significantly differential proteins were identified between fresh and cryoperservated sperm: 98 proteins decreased and 76 proteins increased in the cryopreservation group. Bioinformatic analysis revealed that metabolic pathways play an important role in cryopreservation, including: propanoate metabolism, glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, and pyruvate metabolism. Four different proteins involved in glycolysis were identified by Western blotting: GPI, LDHB, ADH5, and PGAM1.
conclusionsOur work will provide valuable information for future investigations and pathological studies involving sperm cryopreservation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.