ArticleBiosensors2019
A Critical Comparison between Flow-through and Lateral Flow Immunoassay Formats for Visual and Smartphone-Based Multiplex Allergen Detection.
Article in Biosensors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Oxidized carbon black nanoparticles as color label in a rapid vertical flow immunoassay for C-reactive protein detection.Analytical and bioanalytical chemistry · 2026Article
- Exosome Biomarker Profiling Using a Paper-Based Vertical Flow Assay.Biosensors · 2025Article
- Development of a rapid and reliable method to simultaneously detect seven food allergens in processed foods using LC-MS/MS.Food chemistry: X · 2024Article
- Vertical Flow Immunoassay Based on Carbon Black Nanoparticles for the Detection of IgG against SARS-CoV-2 Spike Protein in Human Serum: Proof-of-Concept.Biosensors · 2023Article
- Development of a simple and reliable LC-MS/MS method to simultaneously detect walnut and almond as specified in food allergen labelling regulations in processed foods.Current research in food science · 2023Article
- Secure Food-Allergen Determination by Combining Smartphone-Based Raw Image Analyses and Liquid Chromatography-Mass Spectrometry for the Quantification of Proteins Contained in Lateral Flow Assays.Analytical chemistry · 2022Article
- Optimization of an Antibody Microarray Printing Process Using a Designed Experiment.ACS omega · 2022Article
- Biosensing Chlorpyrifos in Environmental Water Samples by a Newly Developed Carbon Nanoparticle-Based Indirect Lateral Flow Assay.Biosensors · 2022Article
- Review
- Discrimination of Single-Nucleotide Variants Based on an Allele-Specific Hybridization Chain Reaction and Smartphone Detection.ACS sensors · 2022Article
- Optimizing Effective Parameters to Enhance the Sensitivity of Vertical Flow Assay for Detection ofBiosensors · 2022Article
- Blind Spot: A Braille Patterned Novel Multiplex Lateral Flow Immunoassay Sensor Array for the Detection of Biothreat Agents.ACS omega · 2021Article
- Disposable Paper-Based Biosensors for the Point-of-Care Detection of Hazardous Contaminations-A Review.Biosensors · 2021Review
- Development of rapid and cost-effective top-loading device for the detection of anti-SARS-CoV-2 IgG/IgM antibodies.Scientific reports · 2021Article
- ASSURED Point-of-Need Food Safety Screening: A Critical Assessment of Portable Food Analyzers.Foods (Basel, Switzerland) · 2021Review
- Hand-Held Diode Laser for On-Site Analysis Using Transportable Mass Spectrometry.Analytical chemistry · 2021Article
- From Smartphone Lateral Flow Immunoassay Screening to Direct MS Analysis: Development and Validation of a Semi-Quantitative Direct Analysis in Real-Time Mass Spectrometric (DART-MS) Approach to the Analysis of Deoxynivalenol.Sensors (Basel, Switzerland) · 2021Article
- Optical Screening Methods for Pesticide Residue Detection in Food Matrices: Advances and Emerging Analytical Trends.Foods (Basel, Switzerland) · 2021Review
- AuNP Coupled Rapid Flow-Through Dot-Blot Immuno-Assay for Enhanced Detection of SARS-CoV-2 Specific Nucleocapsid and Receptor Binding Domain IgG.International journal of nanomedicine · 2021Article
- Increasing the packing density of assays in paper-based microfluidic devices.Biomicrofluidics · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
(1) Background: The lack of globally standardized allergen labeling legislation necessitates consumer-focused multiplexed testing devices. These should be easy to operate, fast, sensitive and robust. (2) Methods: Herein, we describe the development of three different formats for multiplexed food allergen detection, namely active and passive flow-through assays, and lateral flow immunoassays with different test line configurations. (3) Results: The fastest assay time was 1 min, whereas even the slowest assay was within 10 min. With the passive flow approach, the limits of detection (LOD) of 0.1 and 0.5 ppm for total hazelnut protein (THP) and total peanut protein (TPP) in spiked buffer were reached, or 1 and 5 ppm of THP and TPP spiked into matrix. In comparison, the active flow approach reached LODs of 0.05 ppm for both analytes in buffer and 0.5 and 1 ppm of THP and TPP spiked into matrix. The optimized LFIA configuration reached LODs of 0.1 and 0.5 ppm of THP and TPP spiked into buffer or 0.5 ppm for both analytes spiked into matrix. The optimized LFIA was validated by testing in 20 different blank and spiked matrices. Using device-independent color space for smartphone analysis, two different smartphone models were used for the analysis of optimized assays.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.