ArticleFree radical biology & medicine2020
Quantification of reactive oxygen species production by the red fluorescent proteins KillerRed, SuperNova and mCherry.
Article in Free radical biology & medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Genetic approaches for targeted oxidative stress.NAR cancer · 2025Review
- Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead.Advanced genetics (Hoboken, N.J.) · 2025Review
- Mitochondrial ROS modulate presynaptic plasticity in the drosophila neuromuscular junction.Redox biology · 2025Article
- DMT1 knockout abolishes ferroptosis induced mitochondrial dysfunction in C. elegans amyloid β proteotoxicity.Free radical biology & medicine · 2024Article
- DMT1 knockout abolishes ferroptosis induced mitochondrial dysfunction inbioRxiv : the preprint server for biology · 2024Article
- Article
- Mechanics limits ecological diversity and promotes heterogeneity in confined bacterial communities.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Reactive oxygen species drive foraging decisions in Caenorhabditis elegans.Redox biology · 2023Article
- Photodynamic augmentation of oncolytic virus therapy for central nervous system malignancies.Cancer letters · 2023Article
- All-optical spatiotemporal mapping of ROS dynamics across mitochondrial microdomains in situ.Nature communications · 2023Article
- Photodegradable by Yellow-Orange Light degFusionRed Optogenetic Module with Autocatalytically Formed Chromophore.International journal of molecular sciences · 2023Article
- Optogenetics at the presynapse.Nature neuroscience · 2022Review
- Defining roles of specific reactive oxygen species (ROS) in cell biology and physiology.Nature reviews. Molecular cell biology · 2022Review
- A reversible mitochondrial complex I thiol switch mediates hypoxic avoidance behavior in C. elegans.Nature communications · 2022Article
- Detailed Imaging of Mitochondrial Transport and Precise Manipulation of Mitochondrial Function with Genetically Encoded Photosensitizers in Adult Drosophila Neurons.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Best practices and tools for reporting reproducible fluorescence microscopy methods.Nature methods · 2021Review
- Genetically Encoded Photosensitizer for Destruction of Protein or Cell Function.Advances in experimental medicine and biology · 2021Review
- Mitochondrial light switches: optogenetic approaches to control metabolism.The FEBS journal · 2020Review
- Photodynamic Activation of Cholecystokinin 1 Receptor with Different Genetically Encoded Protein Photosensitizers and from Varied Subcellular Sites.Biomolecules · 2020Article
- Rational Design of Bioavailable Photosensitizers for Manipulation and Imaging of Biological Systems.Cell chemical biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescent proteins can generate reactive oxygen species (ROS) upon absorption of photons via type I and II photosensitization mechanisms. The red fluorescent proteins KillerRed and SuperNova are phototoxic proteins engineered to generate ROS and are used in a variety of biological applications. However, their relative quantum yields and rates of ROS production are unclear, which has limited the interpretation of their effects when used in biological systems. We cloned and purified KillerRed, SuperNova, and mCherry - a related red fluorescent protein not typically considered a photosensitizer - and measured the superoxide (O
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.