ArticlePlant cell reports2020
Identification and functional characterization of squalene epoxidases and oxidosqualene cyclases from Tripterygium wilfordii.
Article in Plant cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 36 citations in OpenAlex.
- Integrated transcriptomic and targeted triterpenoid profiling reveals key enzymes in triterpenoid biosynthesis of Oplopanax elatus.Scientific reports · 2026Article
- Integrated metabolomics and transcriptomics investigate the bulb-specific biosynthesis of medicinal steroidal alkaloids in Fritillaria taipaiensis.BMC plant biology · 2026Article
- Integrative analysis of metabolite and transcriptome reveals the biosynthetic pathway and candidate genes for iridoid glycoside biosynthesis inFrontiers in plant science · 2025Article
- Genome-Wide Identification and Characterization ofLife (Basel, Switzerland) · 2024Article
- Genome-wide identification of oxidosqualene cyclase genes regulating natural rubber in Taraxacum kok-saghyz.Planta · 2024Article
- Article
- Identification of squalene epoxidase in triterpenes biosynthesis in Poria cocos by molecular docking and CRISPR-Cas9 gene editing.Microbial cell factories · 2024Article
- Heterologous expression of Chlorophytum borivilianum Squalene epoxidase in tobacco modulates stigmasterol production and alters vegetative and reproductive growth.Plant cell reports · 2023Article
- Extraction of Squalene From Tea Leaves (Frontiers in nutrition · 2022Article
- Advances in Enhanced Menaquinone-7 Production FromFrontiers in bioengineering and biotechnology · 2021Review
- Regulation of Brassinosteroid Homeostasis in Higher Plants.Frontiers in plant science · 2020Review
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
key messageWe cloned two squalene epoxidases and five oxidosqualene cyclases, and identified their function using CRISPR/Cas9 tool and yeast heterologous expression. Triterpenes are the main active ingredients of Tripterygium wilfordii Hook.f., a traditional Chinese medicinal plant with many encouraging preclinical applications. However, the biosynthetic pathways of triterpenes in this plant are poorly understood. Here, we report on the isolation and identification of two squalene epoxidases (SQE6 and SQE7) and five oxidosqualene cyclases (OSC4-8) from T. wilfordii. Yeast complementation assays showed that TwSQE6 and TwSQE7 can functionally complement an erg1 yeast mutant that was constructed using the CRISPR/Cas9 system. The putative OSC genes were functionally characterized by heterologous expression in yeast. GC/MS analysis of the fermentation products of the transgenic yeast showed that both TwOSC4 and TwOSC6 are cycloartenol synthases, while TwOSC8 is a β-amyrin synthase. The discovery of these genes expands our knowledge of key enzymes in triterpenoid biosynthesis, and provides additional target genes for increasing the production of triterpenes in T. wilfordii tissue cultures by disrupting competing pathways, or in chassis cells by reconstituting the triterpenoid biosynthetic pathway.
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