Evidence map›Paper›PMID 31837261›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2019

The Effects of Orientin on Proliferation and Apoptosis of T24 Human Bladder Carcinoma Cells Occurs Through the Inhibition of Nuclear Factor-kappaB and the Hedgehog Signaling Pathway.

Fenghao Tian, Ming Tong, Zizhi Li, Weichao Huang, Yanyang Jin, Qingfei Cao, Xiaoli Zhou, Guangquan Tong

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 32 citations in OpenAlex.

  1. Article
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  8. Article
  9. Review
  10. Article
  11. Review
  12. Total Flavonoids inInternational journal of molecular sciences · 2023
    Article
  13. Article
  14. Polyphenolic Composition ofMolecules (Basel, Switzerland) · 2023
    Article
  15. Review
  16. Article
  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Fenghao TianJinzhou Medical University, Jinzhou, Liaoning, China (mainland).
Ming TongDepartment of Urological Surgery, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China (mainland).
Zizhi LiDepartment of Urological Surgery, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China (mainland).
Weichao HuangDepartment of Urological Surgery, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China (mainland).
Yanyang JinDepartment of Urological Surgery, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China (mainland).
Qingfei CaoDepartment of Urological Surgery, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China (mainland).
Xiaoli ZhouJinzhou Medical University, Jinzhou, Liaoning, China (mainland).
Guangquan TongDepartment of Urological Surgery, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China (mainland).
Jinzhou Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND Orientin is a flavone isolated from medicinal plants used in traditional Chinese medicine (TCM), which suppresses the growth of cancer cells in vitro. The effects of orientin in bladder cancer cells remains unknown. This study aimed to investigate the effect of orientin on proliferation and apoptosis of T24 human transitional cell bladder carcinoma cells in vitro in the presence of an agonist and an inhibitor of nuclear factor-kappaB (NF-kappaB). MATERIAL AND METHODS T24 cells were cultured and divided into four study groups: an untreated control group; a group treated with 100 μM orientin; a group treated with 100 μM orientin with NF-kappaB agonist, phorbol 12-myristate 13-acetate (PMA); and a group treated with 100 μM orientin and the NF-kappaB inhibitor, IkappaBalpha. The MTT assay was performed to assess cell viability, and flow cytometry evaluated the cell cycle. The expression of proteins in the Hedgehog signaling pathway and inflammatory cytokines were determined by Western blot and enzyme-linked immunosorbent assay (ELISA). RESULTS Orientin inhibited the proliferation of T24 cells, caused cell cycle arrest, reduced cell viability, and inhibited the expression of inflammatory mediators. Treatment of T24 cells with orientin inhibited the expression of NF-kappaB and components of the Hedgehog signaling pathway, and the NF-kappaB agonist, PMA, reversed these effects. CONCLUSIONS Treatment of T24 human bladder carcinoma cells in vitro with orientin inhibited cell proliferation and promoted cell apoptosis by suppressing the Hedgehog signaling pathway and NF-kappaB.

Indexed as

ApoptosisCell CycleCell Cycle CheckpointsCell Line, TumorCell ProliferationCell SurvivalChinaFlavonoidsGlucosidesHedgehog ProteinsHumansI-kappa B ProteinsMedicine, Chinese TraditionalNF-kappa BSignal TransductionUrinary BladderFlavonoidsGlucosidesHedgehog ProteinsI-kappa B ProteinsNF-kappa Borientin

Identifiers

PMID31837261
PMCPMC6929549
OpenAlexW2996182043

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.