ArticleScientific reports2019
Manipulating rod-shaped bacteria with optical tweezers.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Challenges and Future Directives of Synthetic Biology in Engineering Plant-Microbe Partnerships for Sustainable Agriculture.Biotechnology and bioengineering · 2026Review
- Stochastic motility energetics reveals cooperative bacterial swarming in optical tweezers.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Optoelectronic Tweezers for Single-Cell Research: Principles, Applications, and Prospects.Cyborg and bionic systems (Washington, D.C.) · 2026Review
- Article
- Optical tweezers for probing the interactions of ZnO and Ag nanoparticles with E. coli.Archives of microbiology · 2024Article
- Robust acoustic trapping and perturbation of single-cell microswimmers illuminate three-dimensional swimming and ciliary coordination.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers.International journal of molecular sciences · 2023Review
- Biomedical Applications of Microfluidic Devices: A Review.Biosensors · 2022Review
- A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices.Micromachines · 2022Review
- Enhanced Signal-to-Noise and Fast Calibration of Optical Tweezers Using Single Trapping Events.Micromachines · 2021Article
- Nanometer optical trap based on stimulated emission in evanescence of a totally reflected Arago spot : Nanometer optical trap for fluorescent nanoparticles.The European physical journal. E, Soft matter · 2020Article
- Transformation of theApplied and environmental microbiology · 2020Article
- Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers.Micromachines · 2020Article
- Optical Tweezers Exploring Neuroscience.Frontiers in bioengineering and biotechnology · 2020Review
- How bacteria recognise and respond to surface contact.FEMS microbiology reviews · 2020Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optical tweezers have great potential in microbiology for holding and manipulating single cells under a microscope. However, the methodology to use optical tweezers for live cell studies is still at its infancy. In this work, we determined suitable parameters for stable trapping of single Escherichia coli bacteria, and identified the upper limits of IR-exposure that can be applied without affecting viability. We found that the maximum tolerable IR-exposure is 2.5-fold higher when employing oscillating instead of stationary optical trapping (20 J and 8 J, respectively). We found that good stability of cells in an oscillating trap is achieved when the effective trap length is 20% larger than the cell length, the oscillation frequency higher than 100 Hz and the trap oriented perpendicular to the medium flow direction. Further, we show, using an IR power just sufficient for stable holding, that bacteria remain viable during at least 30 min of holding in an oscillating trap. In this work, we established a method for long-term stable handling of single E. coli cells using optical tweezers. This work will pave the way for future use of optical tweezers in microbiology.
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Registered trials
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