ArticleScientific reports2019
Metabolomic Profiles of Bovine Mammary Epithelial Cells Stimulated by Lipopolysaccharide.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 21 citations in OpenAlex.
- The Dry Secretion Metabolome: LC-MS Profiling Distinguishes Subclinical Mastitis from Healthy Udder Quarters Across the Dry Period in Dairy Cows.Veterinary sciences · 2026Article
- Correlation between oxidative stress and inflammation with metabolomics profile in skeletal muscle of ageing animal model and its modulation by tocotrienol-rich fraction.British journal of biomedical science · 2026Article
- Vitamin D metabolites reshape bovine mammary epithelial responses relevant to mastitis through host-directed mechanisms.Frontiers in cellular and infection microbiology · 2026Article
- NovelFrontiers in immunology · 2025Article
- PTGS2/GRP78 Activation Triggers Endoplasmic Reticulum Stress Leading to Lipid Metabolism Disruption and Cell Apoptosis, Exacerbating Damage in Bovine Mastitis.Biomolecules · 2024Article
- Bovine neutrophils stimulated with Streptococcus uberis induce neutrophil extracellular traps, and cause cytotoxicity and transcriptional upregulation of inflammatory cytokine genes in bovine mammary epithelial cells.The Journal of veterinary medical science · 2024Article
- Effects of Glucose Levels on Inflammation and Amino Acid Utilization in Lipopolysaccharide-Induced Bovine Mammary Epithelial Cells.Animals : an open access journal from MDPI · 2023Article
- Differential mRNA Expression Profiling Reveals the Role of MiR-375 in Inflammation of Bovine Mammary Epithelial Cells.Animals : an open access journal from MDPI · 2022Article
- Screening for broad-spectrum antimicrobial endophytes fromFrontiers in plant science · 2022Article
- Regularity of Toll-Like Receptors in Bovine Mammary Epithelial Cells Induced byFrontiers in veterinary science · 2022Article
- The Bio-Persistence of Reversible Inflammatory, Histological Changes and Metabolic Profile Alterations in Rat Livers after Silver/Gold Nanorod Administration.Nanomaterials (Basel, Switzerland) · 2021Article
- Single-cell RNA sequencing of freshly isolated bovine milk cells and cultured primary mammary epithelial cells.Scientific data · 2021Article
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Authors and funding
12 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Bovine mammary epithelial cells (bMECs) are the main cells of the dairy cow mammary gland. In addition to their role in milk production, they are effector cells of mammary immunity. However, there is little information about changes in metabolites of bMECs when stimulated by lipopolysaccharide (LPS). This study describes a metabolomics analysis of the LPS-stimulated bMECs to provide a basis for the identification of potential diagnostic screening biomarkers and possible treatments for bovine mammary gland inflammation. In the present study, bMECs were challenged with 500 ng/mL LPS and samples were taken at 0 h, 12 h and 24 h post stimulation. Metabolic changes were investigated using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF MS) with univariate and multivariate statistical analyses. Clustering and metabolic pathway changes were established by MetaboAnalyst. Sixty-three differential metabolites were identified, including glycerophosphocholine, glycerol-3-phosphate, L-carnitine, L-aspartate, glutathione, prostaglandin G2, α-linolenic acid and linoleic acid. They were mainly involved in eight pathways, including D-glutamine and D-glutamic acid metabolism; linoleic acid metabolism; α-linolenic metabolism; and phospholipid metabolism. The results suggest that bMECs are able to regulate pro-inflammatory, anti-inflammatory, antioxidation and energy-producing related metabolites through lipid, antioxidation and energy metabolism in response to inflammatory stimuli.
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