ArticleNanomaterials (Basel, Switzerland)2019
Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy.
Article in Nanomaterials (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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Who cites it
5 citing papers in PubMed, 15 citations in OpenAlex.
- In vitro dosimetry of multi-walled carbon nanotubes on human bronchial epithelial cells (BEAS-2B) using UV-Vis-NIR spectrophotometry and chemical digestion.Analytical and bioanalytical chemistry · 2026Article
- 2D Hexagonal Boron Nitride (h-BN) and 1D Boron Nitride Nanotubes (BNNTs): Distinct Effects at the Cellular Level in Fish Cell Lines.Journal of xenobiotics · 2025Article
- Removal of Carbon Nanotubes from Aqueous Solutions by Sodium Hypochlorite: Effects of Treatment Conditions.Toxics · 2021Article
- Femtosecond pulsed laser microscopy: a new tool to assess the in vitro delivered dose of carbon nanotubes in cell culture experiments.Particle and fibre toxicology · 2021Article
- Carbon nanomaterial-derived lung burden analysis using UV-Vis spectrophotometry and proteinase K digestion.Particle and fibre toxicology · 2020Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 3 countries.
Funding
Abstract
The overt hazard of carbon nanotubes (CNTs) is often assessed using in vitro methods, but determining a dose-response relationship is still a challenge due to the analytical difficulty of quantifying the dose delivered to cells. An approach to accurately quantify CNT doses for submerged in vitro adherent cell culture systems using UV-VIS-near-infrared (NIR) spectroscopy is provided here. Two types of multi-walled CNTs (MWCNTs), Mitsui-7 and Nanocyl, which are dispersed in protein rich cell culture media, are studied as tested materials. Post 48 h of CNT incubation, the cellular fractions are subjected to microwave-assisted acid digestion/oxidation treatment, which eliminates biological matrix interference and improves CNT colloidal stability. The retrieved oxidized CNTs are analyzed and quantified using UV-VIS-NIR spectroscopy. In vitro imaging and quantification data in the presence of human lung epithelial cells (A549) confirm that up to 85% of Mitsui-7 and 48% for Nanocyl sediment interact (either through internalization or adherence) with cells during the 48 h of incubation. This finding is further confirmed using a sedimentation approach to estimate the delivered dose by measuring the depletion profile of the CNTs.
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Registered trials
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