Evidence map›Paper›PMID 31835532›Full record

ArticleInternational journal of molecular sciences2019

Influence of Lytic Polysaccharide Monooxygenase Active Site Segments on Activity and Affinity.

Christophe V F P Laurent, Peicheng Sun, Stefan Scheiblbrandner, Florian Csarman, Pietro Cannazza, Matthias Frommhagen, Willem J H van Berkel, Chris Oostenbrink, Mirjam A Kabel, Roland Ludwig

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Exosome-based immunotherapy in hepatocellular carcinoma.Clinical and experimental medicine · 2025
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Carbohydrate-binding modules enhance HThe Journal of biological chemistry · 2024
    Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Plastid Transformation: New Challenges in the Circular Economy Era.International journal of molecular sciences · 2022
    Review
  18. Comparison of Six Lytic Polysaccharide Monooxygenases fromApplied and environmental microbiology · 2022
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Christophe V F P LaurentBiocatalysis and Biosensing Laboratory, Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria.ORCID 0000-0002-9112-6981
Peicheng SunLaboratory of Food Chemistry, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.
Stefan ScheiblbrandnerBiocatalysis and Biosensing Laboratory, Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria.
Florian CsarmanBiocatalysis and Biosensing Laboratory, Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria.
Pietro CannazzaBiocatalysis and Biosensing Laboratory, Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria.
Matthias FrommhagenLaboratory of Food Chemistry, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.ORCID 0000-0002-0373-6094
Willem J H van BerkelLaboratory of Food Chemistry, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.ORCID 0000-0002-6551-2782
Chris OostenbrinkInstitute of Molecular Modeling and Simulation, Department of Material Sciences and Process Engineering BOKU-University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria.ORCID 0000-0002-4232-2556
Mirjam A KabelLaboratory of Food Chemistry, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands.
Roland LudwigBiocatalysis and Biosensing Laboratory, Department of Food Science and Technology, BOKU-University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria.ORCID 0000-0002-5058-5874

Funding

Austrian Science Fund W1224Austrian Science Fund FWF W 1224European Research Council 726396
6 · The paper itself

Abstract

In past years, new lytic polysaccharide monooxygenases (LPMOs) have been discovered as distinct in their substrate specificity. Their unconventional, surface-exposed catalytic sites determine their enzymatic activities, while binding sites govern substrate recognition and regioselectivity. An additional factor influencing activity is the presence or absence of a family 1 carbohydrate binding module (CBM1) connected via a linker to the C-terminus of the LPMO. This study investigates the changes in activity induced by shortening the second active site segment (Seg2) or removing the CBM1 from

Indexed as

Binding SitesCarboxymethylcellulose SodiumCatalytic DomainCelluloseFungal ProteinsGlucansMixed Function OxygenasesNeurospora crassaSequence DeletionSurface Plasmon ResonanceXylansCarboxymethylcellulose SodiumCelluloseFungal ProteinsGlucansMixed Function Oxygenasesrayon, purifiedXylansxyloglucanenzyme engineeringlytic polysaccharide monooxygenasephylogenetic analysisregioselectivitysubstrate bindingsubstrate specificity

Identifiers

PMID31835532
PMCPMC6940765

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.