Evidence map›Paper›PMID 31835034›Full record

ArticleCell2019

Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice.

Vincent Villette, Mariya Chavarha, Ivan K Dimov, Jonathan Bradley, Lagnajeet Pradhan, Benjamin Mathieu, Stephen W Evans, Simon Chamberland, Dongqing Shi, Renzhi Yang and 10 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 221 papers.

0numbers the graph read from it
0cells of the map it votes in
221citing papers in PubMed
22.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

221 citing papers in PubMed, 346 citations in OpenAlex.

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  9. Targeting Supramolecular Active Complexes of NaAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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161 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors at 9 institutions in 4 countries.

Vincent VilletteInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France.
Mariya ChavarhaDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Ivan K DimovDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA; CNC Program, Stanford University, Stanford, CA 94305, USA.
Jonathan BradleyInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France.
Lagnajeet PradhanDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA; CNC Program, Stanford University, Stanford, CA 94305, USA.
Benjamin MathieuInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France.
Stephen W EvansDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA.
Simon ChamberlandDepartment of Psychiatry and Neuroscience, CERVO Brain Research Centre, Université Laval, Quebec City, QC G1J 2G3, Canada.
Dongqing ShiDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA; School of Life Sciences, University of Science and Technology of China, Hefei 230026, China.
Renzhi YangDepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USA; Biology PhD Program, Stanford University, Stanford, CA 94305, USA.
Benjamin B KimDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Annick AyonInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France.
Abdelali JalilUniversité de Paris, SPPIN - Saints-Pères Paris Institute for the Neurosciences, CNRS, Paris F-75006, France.
François St-PierreDepartment of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA.
Mark J SchnitzerCNC Program, Stanford University, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Guoqiang BiSchool of Life Sciences, University of Science and Technology of China, Hefei 230026, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai 20031, China.
Katalin TothDepartment of Psychiatry and Neuroscience, CERVO Brain Research Centre, Université Laval, Quebec City, QC G1J 2G3, Canada.
Jun DingDepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
Stéphane DieudonnéInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris 75005, France. Electronic address: dieudon@biologie.ens.fr.
Michael Z LinDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. Electronic address: mzlin@stanford.edu.
Stanford University · USUniversité Paris Sciences et Lettres · FRUniversité Laval · CABaylor College of Medicine · USCenter for Excellence in Brain Science and Intelligence Technology · CNCentre National de la Recherche Scientifique · FRÉcole Normale Supérieure - PSL · FRHoward Hughes Medical Institute · USInserm · FR

Funding

Training Program in Basic NeuroscienceT32MH020016 · NIMH · STANFORD UNIVERSITY · PI Justin L Gardner, Merritt C Maduke · 1997 to 2026
$16.2M
VECTOR COREP30NS069375 · NINDS · STANFORD UNIVERSITY · PI SHAMLOO, MEHRDAD · 2011 to 2019
$6.3M
Bringing laser focus to voltage imaging: Enhanced indicators and advanced scanning methods for two-photon recording of dense networks in vivoU01NS103464 · NINDS · STANFORD UNIVERSITY · PI DIEUDONNE, STEPHANE, LIN, MICHAEL Z. · 2017 to 2019
$2.9M
Revealing circuit control of neuronal excitation with next-generation voltage indicatorsRF1MH114105 · NIMH · STANFORD UNIVERSITY · PI CLANDININ, THOMAS ROBERT, LIN, MICHAEL Z. · 2017 to 2017
$2.9M
Protein voltage sensors: kilohertz imaging of neural dynamics in behaving animalsU01NS090600 · NINDS · STANFORD UNIVERSITY · PI LIN, MICHAEL Z., SCHNITZER, MARK J · 2014 to 2016
$2.8M
Howard Hughes Medical InstituteNIMH NIH HHS RF1 MH114105NIMH NIH HHS T32 MH020016NINDS NIH HHS P30 NS069375NINDS NIH HHS U01 NS090600NINDS NIH HHS U01 NS103464
6 · The paper itself

Abstract

Optical interrogation of voltage in deep brain locations with cellular resolution would be immensely useful for understanding how neuronal circuits process information. Here, we report ASAP3, a genetically encoded voltage indicator with 51% fluorescence modulation by physiological voltages, submillisecond activation kinetics, and full responsivity under two-photon excitation. We also introduce an ultrafast local volume excitation (ULoVE) method for kilohertz-rate two-photon sampling in vivo with increased stability and sensitivity. Combining a soma-targeted ASAP3 variant and ULoVE, we show single-trial tracking of spikes and subthreshold events for minutes in deep locations, with subcellular resolution and with repeated sampling over days. In the visual cortex, we use soma-targeted ASAP3 to illustrate cell-type-dependent subthreshold modulation by locomotion. Thus, ASAP3 and ULoVE enable high-speed optical recording of electrical activity in genetically defined neurons at deep locations during awake behavior.

Indexed as

Theta RhythmWakefulnessAction PotentialsAnimalsBrainCells, CulturedCHO CellsCricetinaeCricetulusFemaleGreen Fluorescent ProteinsGTPase-Activating ProteinsHEK293 CellsHumansMaleMiceGreen Fluorescent ProteinsGTPase-Activating ProteinsPhosphoric Monoester Hydrolasesaction potentialASAP3electrophysiologyelectroporationGEVIRAMPtheta oscillationtwo-photonULoVEvoltage imaging

Identifiers

PMID31835034
PMCPMC6941988
OpenAlexW2996619516

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.