ArticleParasitology research2020
Molecular method for the semiquantitative identification of gastrointestinal nematodes in domestic ruminants.
Article in Parasitology research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 30 citations in OpenAlex.
- Serological detection ofOpen veterinary journal · 2025Article
- Comparison of diagnostic methods for assessment of Ostertagia ostertagi exposure in Norwegian dairy herds.Acta veterinaria Scandinavica · 2023Article
- Prevalence and molecular identification of gastrointestinal nematodes in Qinghai-Tibetan Plateau of China.Veterinary medicine and science · 2023Article
- High diversity and sharing of strongylid nematodes in humans and great apes co-habiting an unprotected area in Cameroon.PLoS neglected tropical diseases · 2023Article
- Low-cost molecular methods to characterise gastrointestinal nematode co-infections of goats in Africa.Parasites & vectors · 2023Article
- Molecular identification ofFrontiers in veterinary science · 2023Article
- Quantitative Monitoring of Selected Groups of Parasites in Domestic Ruminants: A Comparative Review.Pathogens (Basel, Switzerland) · 2021Review
- The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays.Parasites & vectors · 2021Article
- The use of high resolution melting analysis of ITS-1 for rapid differentiation of parasitic nematodes Haemonchus contortus and Ashworthius sidemi.Scientific reports · 2020Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Standard diagnostic methods currently in use for the identification of helminth infections in ruminants are based on the morphological analysis of immature and adult stages of parasites. This paper describes a method for the semiquantitative identification of nematodes, mainly Trichostrongyloidea, at species-level resolution. The method is based on amplification and fragment analysis followed by minisequencing of the ITS-2 region (internal transcribed spacer 2) of the ribosomal DNA of parasite eggs or larvae. This method allows for the identification of seven genera (Chabertia, Cooperia, Haemonchus, Oesophagostomum, Ostertagia, Teladorsagia, and Trichostrongylus) and 12 species (Chabertia ovina, Cooperia curticei, Cooperia punctata, Cooperia oncophora/Cooperia surnabada, Haemonchus contortus, Haemonchus placei, Haemonchus longistipes, Oesophagostomum asperum, Oesophagostomum radiatum, Ostertagia ostertagi, Trichostrongylus axei, and Trichostrongylus colubriformis) of infectious nematodes of domestic ruminants. The concordance between the morphological and molecular analyses in the detection of genera ranged from 0.84 to 0.99, suggesting the proposed detection method is specific, semiquantitative, less laborious, and highly cost-efficient.
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