ArticleThe Journal of cell biology2020
C. elegans pronuclei fuse after fertilization through a novel membrane structure.
Article in The Journal of cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- The centriculum, a membrane reticulum that surrounds Caenorhabditis elegans centrosomes, might serve as a microtubule filter.Journal of cell science · 2026Article
- Getting nuclear size just right - emerging mechanisms regulating nuclear scaling and morphology.Journal of cell science · 2026Review
- Histone Modifications in the Cell Cycle ofEpigenomes · 2026Review
- The Drosophila nucleoporin ELYS is required for parental chromosome arrangement at fertilization.G3 (Bethesda, Md.) · 2025Article
- Mechanisms of nuclear pore complex disassembly by the mitotic Polo-like kinase 1 (PLK-1) inScience advances · 2023Article
- Volume EM: a quiet revolution takes shape.Nature methods · 2023Article
- A membrane reticulum, the centriculum, affects centrosome size and function in Caenorhabditis elegans.Current biology : CB · 2023Article
- Article
- Cortical microtubule pulling forces contribute to the union of the parental genomes in theeLife · 2022Article
- Coupling lipid synthesis with nuclear envelope remodeling.Trends in biochemical sciences · 2022Review
- A versatile enhanced freeze-substitution protocol for volume electron microscopy.Frontiers in cell and developmental biology · 2022Article
- An RNAi screen for genes that affect nuclear morphology in Caenorhabditis elegans reveals the involvement of unexpected processes.G3 (Bethesda, Md.) · 2021Article
- Asymmetric nuclear division in neural stem cells generates sibling nuclei that differ in size, envelope composition, and chromatin organization.Current biology : CB · 2021Article
- A Workflow for High-pressure Freezing and Freeze Substitution of theBio-protocol · 2021Article
- Review
- Cryo-fluorescence microscopy of high-pressure frozen C. elegans enables correlative FIB-SEM imaging of targeted embryonic stages in the intact worm.Methods in cell biology · 2021Article
- PLK-1 promotes the merger of the parental genome into a single nucleus by triggering lamina disassembly.eLife · 2020Article
- Molecular genetics of maternally-controlled cell divisions.PLoS genetics · 2020Article
- Nucleus structure and dynamics.Molecular biology of the cell · 2020Article
- PLK-1 Regulation of Asymmetric Cell Division in the EarlyFrontiers in cell and developmental biology · 2020Review
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7 authors.
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Abstract
After fertilization, parental genomes are enclosed in two separate pronuclei. In Caenorhabditis elegans, and possibly other organisms, when the two pronuclei first meet, the parental genomes are separated by four pronuclear membranes. To understand how these membranes are breached to allow merging of parental genomes we used focused ion beam scanning electron microscopy (FIB-SEM) to study the architecture of the pronuclear membranes at nanometer-scale resolution. We find that at metaphase, the interface between the two pronuclei is composed of two membranes perforated by fenestrations ranging from tens of nanometers to several microns in diameter. The parental chromosomes come in contact through one of the large fenestrations. Surrounding this fenestrated, two-membrane region is a novel membrane structure, a three-way sheet junction, where the four membranes of the two pronuclei fuse and become two. In the plk-1 mutant, where parental genomes fail to merge, these junctions are absent, suggesting that three-way sheet junctions are needed for formation of a diploid genome.
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