ArticleScientific reports2019
Screening of biomarkers of drug resistance or virulence in ESCAPE pathogens by MALDI-TOF mass spectrometry.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- AI-powered rapid detection of multidrug-resistantBioinformatics advances · 2026Article
- Differences and fingerprints of ESBL-producing E. coli from chicken faeces.Scientific reports · 2025Article
- A pilot study on MALDI-TOF MS-based discrimination of Enterococcus faecalis and Lactococcus spp. isolated from bovine milk samples.World journal of microbiology & biotechnology · 2025Article
- Automated web-based typing of Clostridioides difficile ribotypes via MALDI-TOF MS.BMC bioinformatics · 2025Article
- Spectral Precision: Recent Advances in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Pathogen Detection and Resistance Profiling.Microorganisms · 2025Review
- Identification of Virulence Genes and Antibiotic Resistance in Extraintestinal PathogenicPathogens (Basel, Switzerland) · 2025Article
- Analytical techniques and molecular platforms for detection and surveillance of antimicrobial resistance: advancements of the past decade.3 Biotech · 2025Review
- Prevalence of difficult-to-treat resistance in ESKAPE pathogens in a third level hospital in Mexico.Infection prevention in practice · 2025Article
- Detection of virulence factors in opportunistic bacteria: advances, challenges, and practical implementation.Frontiers in microbiology · 2025Review
- Resolution of MALDI-TOF compared to whole genome sequencing for identification ofFrontiers in microbiology · 2025Article
- Anticancer and Antioxidant Activities of Rhizospheric Soil Bacteria ofInternational journal of microbiology · 2025Article
- Genetic Characterization of Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2024Article
- Contribution of MALDI-TOF mass spectrometry and machine learning including deep learning techniques for the detection of virulence factors of Clostridioides difficile strains.Microbial biotechnology · 2024Article
- MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology Laboratory.Microorganisms · 2024Review
- Diagnostic Guidance for C. difficile Infections.Advances in experimental medicine and biology · 2024Article
- Biomarkers of caspofungin resistance inVirulence · 2022Article
- Molecular phenotyping approaches for the detection and monitoring of carbapenem-resistant Enterobacteriaceae by mass spectrometry.Journal of mass spectrometry and advances in the clinical lab · 2022Review
- Emerging vancomycin-non susceptible coagulase negative Staphylococci associated with skin and soft tissue infections.Annals of clinical microbiology and antimicrobials · 2022Article
- Differentiation ofMicroorganisms · 2022Article
- Recent Developments in Phenotypic and Molecular Diagnostic Methods for Antimicrobial Resistance Detection inDiagnostics (Basel, Switzerland) · 2022Review
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid identification and characterisation of drug-resistant bacterial pathogens have an important role in diagnostic and antimicrobial stewardship. Response time in the diagnosis of not only the etiological agent but also in antimicrobial susceptibility results is of utmost importance in patient treatment. In this study, matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF) mass spectrometry (MS) was used to screen for biomarkers of ESCAPE (vancomycin-resistant Enterococcus faecium, methicillin-resistant Staphylococcus aureus, hypervirulent NAP1/ribotype 027 Clostridioides [Clostridium] difficile, multidrug resistant Acinetobacter baumannii, multidrug resistant Pseudomonas aeruginosa, and carbapenem-resistant Enterobacteriaceae) pathogens to predict antimicrobial resistance or hypervirulence. Several biomarkers of drug-resistant genotypes in S. aureus, A. baumannii, P. aeruginosa, and K. pneumoniae, as well as hypervirulence in C. difficile, were detected. The fastest possible susceptibility testing with MALDI-TOF MS is simultaneous detection of a characteristic drug-resistant peak and species identification in the same spectra generated in routine processing. According to our approach, resistance or virulence biomarker peaks can be identified while performing routine microbiology analysis, and no additional assays nor prolonged incubation time is needed. Outstanding biomarker peaks detected in our study should be further analysed by additional methods to identify the specific proteins involved.
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