ArticleScientific reports2019
SureSelect targeted enrichment, a new cost effective method for the whole genome sequencing of Candidatus Liberibacter asiaticus.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Article
- A Review of Probe-Based Enrichment Methods to Inform Plant Virus Diagnostics.International journal of molecular sciences · 2024Review
- Target enrichment improves culture-independent detection ofMicrobial genomics · 2024Article
- Comparing molecular and computational approaches for detecting viral integration of AAV gene therapy constructs.Molecular therapy. Methods & clinical development · 2023Article
- Whole genome sequencing ofFrontiers in microbiology · 2023Article
- Multilocus sequence typing of diverse phytoplasmas using hybridization probe-based sequence capture provides high resolution strain differentiation.Frontiers in microbiology · 2022Article
- Full Molecular Typing of Neisseria meningitidis Directly from Clinical Specimens for Outbreak Investigation.Journal of clinical microbiology · 2020Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Huanglongbing (HLB) is a worldwide deadly citrus disease caused by the phloem-limited bacteria 'Candidatus Liberibacter asiaticus' (CLas) vectored by Asian citrus psyllids. In order to effectively manage this disease, it is crucial to understand the relationship among the bacterial isolates from different geographical locations. Whole genome sequencing approaches will provide more precise molecular characterization of the diversity among populations. Due to the lack of in vitro culture, obtaining the whole genome sequence of CLas is still a challenge, especially for medium to low titer samples. Hundreds of millions of sequencing reads are needed to get good coverage of CLas from an HLB positive citrus sample. In order to overcome this limitation, we present here a new method, Agilent SureSelect
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