Evidence map›Paper›PMID 31831525›Full record

ArticleJournal of lipid research2020

Functional recombinant apolipoprotein A5 that is stable at high concentrations at physiological pH.

Mark Castleberry, Xenia Davis, Min Liu, Thomas B Thompson, Patrick Tso, W Sean Davidson

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of lipid research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Mark CastleberryDepartments of Molecular Genetics, Biochemistry, and Microbiology University of Cincinnati College of Medicine, Cincinnati, OH.
Xenia DavisPathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH.
Min LiuPathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH.
Thomas B ThompsonDepartments of Molecular Genetics, Biochemistry, and Microbiology University of Cincinnati College of Medicine, Cincinnati, OH.ORCID 0000-0002-7041-5047
Patrick TsoPathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH.
W Sean DavidsonPathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OH davidswm@ucmail.uc.edu.ORCID 0000-0003-2756-2989
University of Cincinnati · USUniversity of Cincinnati Medical Center · US

Funding

Understanding Cardiovascular Disease MechanismsT32HL125204 · NHLBI · CINCINNATI CHILDRENS HOSP MED CTR · PI Evangelia G Kranias, Jeffery D Molkentin · 2015 to 2026
$3.4M
Structural/functional characterization of TGFβ superfamily signaling and regulationR35GM134923 · NIGMS · UNIVERSITY OF CINCINNATI · PI THOMPSON, THOMAS B · 2020 to 2024
$2.6M
NHLBI NIH HHS T32 HL125204NIGMS NIH HHS R35 GM134923
6 · The paper itself

Abstract

APOA5 is a low-abundance exchangeable apolipoprotein that plays critical roles in human triglyceride (TG) metabolism. Indeed, aberrations in the plasma concentration or structure of APOA5 are linked to hypertriglyceridemia, hyperchylomicronemia, myocardial infarction risk, obesity, and coronary artery disease. While it has been successfully produced at low yield in bacteria, the resulting protein had limitations for structure-function studies due to its low solubility under physiological buffer conditions. We hypothesized that the yield and solubility of recombinant APOA5 could be increased by:

Indexed as

AnimalsApolipoprotein A-VEscherichia coliHumansHydrogen-Ion ConcentrationMaleMiceMice, Inbred C57BLRecombinant ProteinsAPOA5 protein, humanApolipoprotein A-VRecombinant Proteinsdyslipidemiaslipid and lipoprotein metabolismlipoproteinstriglycerides

Identifiers

PMID31831525
PMCPMC6997596
OpenAlexW2995051368

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.