ArticlePLoS genetics2019
Coacting enhancers can have complementary functions within gene regulatory networks and promote canalization.
Article in PLoS genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 21 citations in OpenAlex.
- Temporal loss ofGenes & development · 2026Article
- Mitotic reactivation and transcriptional bursting govern transcriptional noise in the early Drosophila embryo.Development (Cambridge, England) · 2026Article
- Inferring chromatin architecture at a single locus through probabilistic in situ DNA localization.Nature communications · 2026Article
- Optogenetic manipulation of nuclear Dorsal reveals temporal requirements and consequences for transcription.Development (Cambridge, England) · 2025Article
- Optogenetic manipulation of nuclear Dorsal reveals temporal requirements and consequences for transcription.bioRxiv : the preprint server for biology · 2024Article
- Target gene responses differ when transcription factor levels are acutely decreased by nuclear export versus degradation.Development (Cambridge, England) · 2024Article
- Notch-Dependent Expression of theGenes · 2024Article
- Target gene responses differ when transcription factor levels are acutely decreased by nuclear export versus degradation.bioRxiv : the preprint server for biology · 2024Article
- Spatial promoter-enhancer hubs in cancer: organization, regulation, and function.Trends in cancer · 2023Review
- Chromatin accessibility in the Drosophila embryo is determined by transcription factor pioneering and enhancer activation.Developmental cell · 2023Article
- Review
- Shadow enhancers mediate trade-offs between transcriptional noise and fidelity.PLoS computational biology · 2023Article
- Shadow enhancers modulate distinct transcriptional parameters that differentially effect downstream patterning events.Development (Cambridge, England) · 2022Article
- Identification of enhancer regulatory elements that direct epicardial gene expression during zebrafish heart regeneration.Development (Cambridge, England) · 2022Article
- Regulatory modules mediating the complex neural expression patterns of the homeobrain gene during Drosophila brain development.Hereditas · 2022Article
- Enhancer redundancy in development and disease.Nature reviews. Genetics · 2021Review
- Precision of tissue patterning is controlled by dynamical properties of gene regulatory networks.Development (Cambridge, England) · 2021Article
- Dynamic patterning by morphogens illuminated by cis-regulatory studies.Development (Cambridge, England) · 2021Review
- Shadow enhancers can suppress input transcription factor noise through distinct regulatory logic.eLife · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Developmental genes are often regulated by multiple enhancers exhibiting similar spatiotemporal outputs, which are generally considered redundantly acting though few have been studied functionally. Using CRISPR-Cas9, we created deletions of two enhancers, brk5' and brk3', that drive similar but not identical expression of the gene brinker (brk) in early Drosophila embryos. Utilizing both in situ hybridization and quantitative mRNA analysis, we investigated the changes in the gene network state caused by the removal of one or both of the early acting enhancers. brk5' deletion generally phenocopied the gene mutant, including expansion of the BMP ligand decapentaplegic (dpp) as well as inducing variability in amnioserosa tissue cell number suggesting a loss of canalization. In contrast, brk3' deletion presented unique phenotypes including dorsal expansion of several ventrally expressed genes and a decrease in amnioserosa cell number. Similarly, deletions were made for two enhancers associated with the gene short-gastrulation (sog), sog.int and sog.dist, demonstrating that they also exhibit distinct patterning phenotypes and affect canalization. In summary, this study shows that similar gene expression driven by coacting enhancers can support distinct, and sometimes complementary, functions within gene regulatory networks and, moreover, that phenotypes associated with individual enhancer deletion mutants can provide insight into new gene functions.
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