ArticleJournal of the American Chemical Society2020
Covalently Tethered Rhodamine Voltage Reporters for High Speed Functional Imaging in Brain Tissue.
Article in Journal of the American Chemical Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- Optimizing multifunctional fluorescent ligands for intracellular labeling.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Membrane potential and feedback dynamics regulate CatSper-mediated progesterone signaling in human sperm.bioRxiv : the preprint server for biology · 2025Article
- Water-Soluble Molecular Wires for Membrane Potential Imaging.Angewandte Chemie (International ed. in English) · 2025Article
- Seeing the Spikes: The Future of Targetable Synthetic Voltage Sensors.ACS chemical neuroscience · 2025Review
- Improved Sensitivity in a Modified Berkeley Red Sensor of Transmembrane Potential.ACS chemical biology · 2024Article
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Optical Estimation of Membrane Potential Values Using Fluorescence Lifetime Imaging Microscopy and Hybrid Chemical-Genetic Voltage Indicators.Bioelectricity · 2024Article
- Orange/far-red hybrid voltage indicators with reduced phototoxicity enable reliable long-term imaging in neurons and cardiomyocytes.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Fluorescence Imaging of Cell Membrane Potential: From Relative Changes to Absolute Values.International journal of molecular sciences · 2023Review
- Multifunctional stimuli-responsive chemogenetic platform for conditional multicolor cell-selective labeling.Chemical science · 2022Article
- Bioorthogonal, Fluorogenic Targeting of Voltage-Sensitive Fluorophores for Visualizing Membrane Potential Dynamics in Cellular Organelles.Journal of the American Chemical Society · 2022Article
- Chemical Targeting of Rhodol Voltage-Sensitive Dyes to Dopaminergic Neurons.ACS chemical neuroscience · 2022Article
- Ketone Incorporation Extends the Emission Properties of the Xanthene Scaffold Beyond 1000 nm.Photochemistry and photobiology · 2022Article
- Recent biomedical advances enabled by HaloTag technology.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2022Article
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- HaloTag Engineering for Enhanced Fluorogenicity and Kinetics with a Styrylpyridium Dye.Chembiochem : a European journal of chemical biology · 2021Article
- Optical probing of local membrane potential with fluorescent polystyrene beads.Biophysical reports · 2021Article
- A silicon-rhodamine chemical-genetic hybrid for far red voltage imaging from defined neurons in brain slice.RSC chemical biology · 2021Article
- The HaloTag as a general scaffold for far-red tunable chemigenetic indicators.Nature chemical biology · 2021Article
- Small Molecule-Protein Hybrid for Voltage Imaging via Quenching of Bioluminescence.ACS sensors · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Voltage-sensitive fluorophores enable the direct visualization of membrane potential changes in living systems. To pair the speed and sensitivity of chemically synthesized fluorescent indicators with cell-type specific genetic methods, we here develop Rhodamine-based Voltage Reporters (RhoVR) that can be covalently tethered to genetically encoded, self-labeling enzymes. These chemical-genetic hybrids feature a photoinduced electron transfer triggered RhoVR voltage-sensitive indicator coupled to a chloroalkane HaloTag ligand through a long, water-soluble polyethylene glycol linker (RhoVR-Halo). When applied to cells, RhoVR-Halo dyes selectively and covalently bind to surface-expressed HaloTag enzyme on genetically modified cells. RhoVR-Halo dyes maintain high voltage sensitivities-up to 34% Δ
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.