ArticleThe Journal of cell biology1988
The reorganization of microfilaments, centrosomes, and microtubules during in vitro small wound reendothelialization.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Integrated analysis of long noncoding RNA and mRNA profiling ox-LDL-induced endothelial dysfunction after atorvastatin administration.Medicine · 2018Trial
- Discovery and Development of Tumor Angiogenesis Assays.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The role of SIRT2 in vascular-related and heart-related diseases: A review.Journal of cellular and molecular medicine · 2021Review
- Water extract of Clinacanthus nutans leaves exhibits in vitro, ex vivo and in vivo anti-angiogenic activities in endothelial cell via suppression of cell proliferation.BMC complementary and alternative medicine · 2018Article
- Alpha-tubulin enhanced renal tubular cell proliferation and tissue repair but reduced cell death and cell-crystal adhesion.Scientific reports · 2016Article
- Cadherins as regulators of neuronal polarity.Cell adhesion & migration · 2015Review
- Evaluation of the antioxidant and melanogenesis inhibitory properties of pracparatum mungo (lu-do huang).Journal of traditional and complementary medicine · 2013Article
- Tumor cell migration in complex microenvironments.Cellular and molecular life sciences : CMLS · 2013Review
- Orientation and function of the nuclear-centrosomal axis during cell migration.Current opinion in cell biology · 2011Review
- A high throughput, interactive imaging, bright-field wound healing assay.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2011Article
- A critical analysis of current in vitro and in vivo angiogenesis assays.International journal of experimental pathology · 2009Review
- Inhibition of ovarian cancer cell metastasis by a fusion polypeptide Tat-ELP.Clinical & experimental metastasis · 2009Article
- Identification of novel pro-migratory, cancer-associated genes using quantitative, microscopy-based screening.PloS one · 2008Article
- A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods.BMC biotechnology · 2004Article
- Endothelial Regulation of Vascular Repair: Role of bFGF in Paracrine Pathways.Endocrine pathology · 1997Article
- Decreased blood flow rate disrupts endothelial repair in vivo.The American journal of pathology · 1996Article
- Postnatal reorganization of actin filaments and differentiation of intercellular boundaries in the rat aortic endothelial cells.Cell and tissue research · 1994Article
- Three patterns of distribution characterize the organization of endothelial microfilaments at aortic flow dividers.Cell and tissue research · 1994Article
- A novel cytoskeletal structure involved in purse string wound closure and cell polarity maintenance.The Journal of cell biology · 1993Article
- Replication of Marburg virus in human endothelial cells. A possible mechanism for the development of viral hemorrhagic disease.The Journal of clinical investigation · 1993Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The repair of small endothelial wounds is an important process by which endothelial cells maintain endothelial integrity. An in vitro wound model system was used in which precise wounds were made in a confluent endothelial monolayer. The repair process was observed by time-lapse cinemicrophotography. Using fluorescence and immunofluorescence microscopy, the cellular morphological events were correlated with the localization and distribution of actin microfilament bundles and vinculin plaques, and centrosomes and their associated microtubules. Single to four-cell wounds underwent closure by cell spreading while wounds seven to nine cells in size closed by initially spreading which was then followed at approximately 1 h after wounding by cell migration. These two processes showed different cytoskeletal patterns. Cell spreading occurred independent of centrosome location. However, centrosome redistribution to the front of the cell occurred as the cells began to elongate and migrate. While the peripheral actin microfilament bundles (i.e., the dense peripheral band) remained intact during cell spreading, they broke down during migration and were associated with a reduction in peripheral vinculin plaque staining. Thus, the major events characterizing the closure of endothelial wounds were precise in nature, followed a specific sequence, and were associated with specific cytoskeletal patterns which most likely were important in maintaining directionality of migration and reducing the adhesion of the cells to their neighbors within the monolayer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.