Evidence map›Paper›PMID 31827193›Full record

ArticleScientific reports2019

First instance of settlement by cryopreserved coral larvae in symbiotic association with dinoflagellates.

Luca Cirino, Zhi-Hong Wen, Kevin Hsieh, Cheng-Liang Huang, Qi Lun Leong, Li-Hsueh Wang, Chii-Shiarng Chen, Jonathan Daly, Sujune Tsai, Chiahsin Lin

Abstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Biobanking biodiversity: recent strategies, challenges, and opportunities.The Journal of reproduction and development · 2026
    Review
  2. Advanced Rewarming Technologies for Cryopreservation.Methods in molecular biology (Clifton, N.J.) · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Luca CirinoDepartment of Marine Biotechnology and Resources, National Sun Yai-sen University, Kaohsiung, Taiwan.
Zhi-Hong WenDepartment of Marine Biotechnology and Resources, National Sun Yai-sen University, Kaohsiung, Taiwan.
Kevin HsiehHe Wei Precision Company Limited, Hsinchu, Taiwan.
Cheng-Liang HuangDepartment of Applied Chemistry, National Chiayi University, Chiayi, Taiwan.
Qi Lun LeongDepartment of Applied Chemistry, National Chiayi University, Chiayi, Taiwan.
Li-Hsueh WangNational Museum of Marine Biology & Aquarium, Pingtung, Taiwan.
Chii-Shiarng ChenDepartment of Marine Biotechnology and Resources, National Sun Yai-sen University, Kaohsiung, Taiwan.
Jonathan DalySmithsonian Conservation Biology Institute, Washington, USA.
Sujune TsaiDepartment of Post Modern Agriculture, Mingdao University, Chang Hua, Taiwan. stsai@mdu.edu.tw.
Chiahsin LinNational Museum of Marine Biology & Aquarium, Pingtung, Taiwan. chiahsin@nmmba.gov.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Coral reefs are suffering on a global scale due to human impacts, thereby necessitating cryopreservation efforts. The objective of this study was to develop a suitable vitrification and laser warming protocol for larvae of the scleractinian coral Seriatopora caliendrum, which inherit their dinoflagellate algal symbionts vertically. Toxicity experiments were conducted with the cryoprotectants (CPAs) ethylene glycol (EG), propylene glycol (PG), dimethyl sulfoxide (DMSO), glycerol (GLY), and methanol (METH; listed in order from least to most toxic), and larvae were subjected to vitrification and laser warming using 2 M EG + 1 M PG and 2 M EG + 1 M DMSO. Vitrification and laser warming (300 V, 10 ms pulse width, 2 mm beam diameter) using a vitrification solution of 2 M EG + 1 M PG, 40% w/v Ficoll, and 10% v/v gold nanobars (GNB) at a final concentration of 1.2 × 10

Indexed as

AnthozoaDinoflagellidaLarvaVitrificationAnimalsCryopreservationCryoprotective AgentsEthylene GlycolPropylene GlycolSymbiosisCryoprotective AgentsEthylene GlycolPropylene Glycol

Identifiers

PMID31827193
PMCPMC6906398

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.