ArticlePloS one2019
Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Characterizing immune perturbations in peripheral blood following the East Palestine, Ohio train derailment.Journal of exposure science & environmental epidemiology · 2026Article
- A context-aware interpretive framework for lymphocyte immunophenotyping by flow cytometry.Frontiers in immunology · 2026Article
- A Tree Perspective on Stick-Breaking Models in Covariate-Dependent Mixtures (with Discussion).Bayesian analysis · 2025Article
- Immunological biomarkers of aging.Journal of immunology (Baltimore, Md. : 1950) · 2025Review
- Association between peripheral blood immunological status and intrathecal inflammatory markers differentiate multiple sclerosis clinical phenotypes.Acta neurologica Belgica · 2024Article
- Age-dependent immune profile in healthy individuals: an original study, systematic review and meta-analysis.Immunity & ageing : I & A · 2024Article
- Cellular and humoral immunogenicity against SARS-CoV-2 vaccination or infection is associated with the memory phenotype of T- and B-lymphocytes in adult allogeneic hematopoietic cell transplant recipients.International journal of hematology · 2024Article
- From Genesis to Old Age: Exploring the Immune System One Cell at a Time with Flow Cytometry.Biomedicines · 2024Review
- Article
- Design and validation of novel flow cytometry panels to analyze a comprehensive range of peripheral immune cells in mice.Frontiers in immunology · 2024Article
- Normative data for paediatric lymphocyte subsets: A pilot study from western India.The Indian journal of medical research · 2023Article
- Fungal Rhinosinusitis Caused by aPathogens (Basel, Switzerland) · 2022Article
- Antibody-mediated prevention of vaginal HIV transmission is dictated by IgG subclass in humanized mice.Science translational medicine · 2022Article
- T cell counts and IL-6 concentration in blood of North African COVID-19 patients are two independent prognostic factors for severe disease and death.Journal of leukocyte biology · 2022Article
- Altered transcription factor targeting is associated with differential peripheral blood mononuclear cell proportions in sarcoidosis.Frontiers in immunology · 2022Article
- Shifting gears: Study of immune system parameters of male habitual marathon runners.Frontiers in immunology · 2022Article
- Could veterinarians be immune to contracting SARS-CoV-2?Central-European journal of immunology · 2022Article
- Serological responses to SARS-CoV-2 following non-hospitalised infection: clinical and ethnodemographic features associated with the magnitude of the antibody response.BMJ open respiratory research · 2021Article
- Lymphocyte subpopulations in Sjögren's syndrome are distinct in anti-SSA-positive patients and related to disease activity.Clinical rheumatology · 2021Article
- Immune cell profiling in the joint following human and murine articular fracture.Osteoarthritis and cartilage · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte subpopulations. To overcome these issues, we defined high-dimensional flow cytometry reference ranges for the healthy human immune system using Human Immunology Project Consortium methodologies after carefully screening 127 subjects deemed healthy through clinical and laboratory testing. We enrolled subjects in the following age cohorts: 18-29 years, 30-39, 40-49, and 50-66 and enrolled cohorts to ensure an even gender distribution and at least 30% non-Caucasians. From peripheral blood mononuclear cells, flow cytometry reference ranges were defined for >50 immune subsets including T-cell (activation, maturation, T follicular helper and regulatory T cell), B-cell, and innate cells. We also developed a web tool for visualization of the dataset and download of raw data. This dataset provides the immunology community with a resource to compare and extract data from rigorously characterized healthy subjects across age groups, gender and race.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.