ArticleFrontiers in microbiology2019
Characterization of the Cultivable Microbiota in Fresh and Stored Mature Human Breast Milk.
Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Beyond just correlation: causal machine learning for the microbiome, from prediction to health policy with econometric tools.Frontiers in microbiology · 2025Pooled it
- A Framework for Evaluation of New Processing Technologies in Human Milk Banking.Comprehensive reviews in food science and food safety · 2025Review
- Viable bacterial communities in freshly pumped human milk and their changes during cold storage conditions.International breastfeeding journal · 2025Observational
- New insights into the cultivability of human milk bacteria from ingestion to digestion and implications for their Immunomodulatory properties.Scientific reports · 2025Article
- Bacterial Community of Breast Milk in Breastfeeding Women Using Culture-Dependent and Culture-Independent Approaches.Journal of microbiology and biotechnology · 2024Article
- Identification and Characterization of Human Breast Milk and Infant Fecal Cultivable Lactobacilli Isolated in Bulgaria: A Pilot Study.Microorganisms · 2024Article
- Bovine milk microbiota: Key players, origins, and potential contributions to early-life gut development.Journal of advanced research · 2024Review
- Seeding and feeding milestones: the role of human milk microbes and oligosaccharides in the temporal development of infant gut microbiota.Gut microbiome (Cambridge, England) · 2024Article
- An optimized culturomics strategy for isolation of human milk microbiota.Frontiers in microbiology · 2024Article
- Human milk microbiota: what did we learn in the last 20 years?Microbiome research reports · 2022Review
- Building Robust Assemblages of Bacteria in the Human Gut in Early Life.Applied and environmental microbiology · 2021Review
- Bacterial Diversity of Breast Milk in Healthy Spanish Women: Evolution from Birth to Five Years Postpartum.Nutrients · 2021Article
- Comparison of Two Approaches for the Metataxonomic Analysis of the Human Milk Microbiome.Frontiers in cellular and infection microbiology · 2021Article
- The Relationship Between Breast Milk Components and the Infant Gut Microbiota.Frontiers in nutrition · 2021Review
- Best Practices for Human Milk Collection for COVID-19 Research.Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine · 2021Review
- State of the Art in the Culture of the Human Microbiota: New Interests and Strategies.Clinical microbiology reviews · 2020Review
- Like mother, like microbe: human milk oligosaccharide mediated microbiome symbiosis.Biochemical Society transactions · 2020Review
- Bioactive Factors in Human Breast Milk Attenuate Intestinal Inflammation during Early Life.Nutrients · 2020Review
- Dietary Prevention of Atopic March in Pediatric Subjects With Cow's Milk Allergy.Frontiers in pediatrics · 2020Review
- The Microbiota of the Human Mammary Ecosystem.Frontiers in cellular and infection microbiology · 2020Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Besides nutritional components, breast milk contains diverse microbes, which may be involved in colonization of the infant gut. Expressed milk is often stored for few days in the refrigerator. The aim of this study was to determine the abundance, prevalence and diversity of facultative and strict anaerobic bacteria using culture-dependent and -independent methods, and to determine changes in milk microbial and chemical composition during storage. Samples of mature breast milk from 21 women were collected 3-6 months post-partum and were analyzed fresh or after anaerobic storage for 6 days at 4°C. The cultivable bacterial population was analyzed using the most probable number (MPN) method or plate counts and different media. The abundance of major bacterial groups was determined using quantitative PCR and 16S rRNA gene sequencing. Lactose, lactate, short chain fatty acids (SCFA) and human milk oligosaccharides (HMO) were analyzed using chromatography techniques. Highest mean viable cell counts were obtained in yeast casitone fatty acids (YCFA) broth supplied with mucin (log 4.2 ± 1.8 cells/ml) and lactose (log 3.9 ± 1.4 cells/ml), or Columbia broth (log 3.0 ± 0.7 cells/ml). Mean total bacterial counts estimated by qPCR was 5.3 ± 0.6 log cells/ml, with
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Registered trials
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