ReviewAndrology2020
In-vitro spermatogenesis through testis modelling: Toward the generation of testicular organoids.
Review in Andrology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed.
- In vitro models for testicular steroidogenesis: current status and future perspectives.Archives of toxicology · 2026Review
- Tuning the Testicular Microenvironment for Enhancing Human Sertoli Cells Maturation and Functionality In Vitro.Advanced healthcare materials · 2026Article
- Testicular organoid models in male reproductive toxicology: advances, applications, and future perspectives.Frontiers in physiology · 2026Article
- Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Decellularized testicular bio-scaffolds driveFrontiers in bioengineering and biotechnology · 2026Article
- Testicular tissue transplantation: from animal models to clinical application.Human reproduction open · 2026Review
- Differential retinoic acid responses across testicular developmentbioRxiv : the preprint server for biology · 2025Article
- Harnessing TGF-β signaling to improve testicular organoid development from dissociated testicular cells.Stem cell research & therapy · 2025Article
- Artificial Gametogenesis and In Vitro Spermatogenesis: Emerging Strategies for the Treatment of Male Infertility.International journal of molecular sciences · 2025Review
- Brain-derived neurotrophic factor and neurotrophic tyrosine receptor kinase-2 in stallion testes: insights into seasonal changes and potential roles in spermatogenesis.Journal of animal science and technology · 2025Article
- GDNF enhances HGF-induced tubulogenesis and organization of Sertoli cell.Journal of assisted reproduction and genetics · 2025Article
- Testicular Cell-Conditioned Chitosan Thermosensitive Hydrogel as an Excellent Substrate for Culture and Proliferation of Spermatogonia Cells.BioMed research international · 2025Article
- Engineering a 3D platform for testis bioengineering: generation and proteomic profiling of decellularized fish testicular scaffolds.Frontiers in bioengineering and biotechnology · 2025Article
- Advances in real-world research on fertility preservation in men with malignant tumours.Frontiers in urology · 2025Review
- Extracellular vesicles in stem cell-based gonadal regeneration: mechanisms, therapeutic potential, and translational challenges.Frontiers in veterinary science · 2025Review
- Generation of Organotypic Testicular Organoids from Rat and Pig Primary Cells in Microwell Culture.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- Advancement and Potential Applications of Epididymal Organoids.Biomolecules · 2024Review
- MicroRNA Analysis of In Vitro Differentiation of Spermatogonial Stem Cells Using a 3D Human Testis Organoid System.Biomedicines · 2024Article
- The effect of epididymosomes on the development of frozen-thawed mouse spermatogonial stem cells after culture in a decellularized testicular scaffold and transplantation into azoospermic mice.Journal of assisted reproduction and genetics · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe testicular organoid concept has recently been introduced in tissue engineering to refer to testicular cell organizations modeling testicular architecture and function. The testicular organoid approach gives control over which and how cells reaggregate, which is not possible in organotypic cultures, thereby extending the applicability of in-vitro spermatogenesis (IVS) systems. However, it remains unclear which culture method and medium allow reassociation of testicular cells into a functional testicular surrogate in-vitro.
objectiveThe aim of this paper is to review the different strategies that have been used in an attempt to create testicular organoids and generate spermatozoa. We want to provide an up-to-date list on culture methodologies and media compositions that have been used and determine their role in regulating tubulogenesis and differentiation of testicular cells. SEARCH
methodA literature search was conducted in PubMed, Web of Science, and Scopus to select studies reporting the reorganization of testicular cell suspensions in-vitro, using the keywords: three-dimensional culture, in-vitro spermatogenesis, testicular organoid, testicular scaffold, and tubulogenesis. Papers published before the August 1, 2019, were selected. OUTCOME: Only a limited number of studies have concentrated on recreating the testicular architecture in-vitro. While some advances have been made in the testicular organoid research in terms of cellular reorganization, none of the described culture systems is adequate for the reproduction of both the testicular architecture and IVS.
conclusionFurther improvements in culture methodology and medium composition have to be made before being able to provide both testicular tubulogenesis and spermatogenesis in-vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.