Evidence map›Paper›PMID 31822287›Full record

ArticleBMC infectious diseases2019

Association of CMV genomic mutations with symptomatic infection and hearing loss in congenital CMV infection.

G Clement Dobbins, Amit Patki, Dongquan Chen, Hemant K Tiwari, Curtis Hendrickson, William J Britt, Karen Fowler, Jake Y Chen, Suresh B Boppana, Shannon A Ross

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC infectious diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.

  1. Pooled it
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  3. Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

G Clement DobbinsDepartment of Pediatrics, The University of Alabama School of Medicine, CHB 116, 1600 6th Avenue South, Birmingham, AL, USA. gclemd@uab.edu.
Amit PatkiDepartment of Biostatistics, The University of Alabama School of Public Health, Birmingham, AL, USA.
Dongquan ChenInformatics Institute, The University of Alabama at Birmingham, Birmingham, AL, USA.
Hemant K TiwariDepartment of Biostatistics, The University of Alabama School of Public Health, Birmingham, AL, USA.
Curtis HendricksonDepartment of Microbiology, The University of Alabama at Birmingham, Birmingham, AL, USA.
William J BrittDepartment of Pediatrics, The University of Alabama School of Medicine, CHB 116, 1600 6th Avenue South, Birmingham, AL, USA.
Karen FowlerDepartment of Pediatrics, The University of Alabama School of Medicine, CHB 116, 1600 6th Avenue South, Birmingham, AL, USA.
Jake Y ChenInformatics Institute, The University of Alabama at Birmingham, Birmingham, AL, USA.
Suresh B BoppanaDepartment of Pediatrics, The University of Alabama School of Medicine, CHB 116, 1600 6th Avenue South, Birmingham, AL, USA.
Shannon A RossDepartment of Pediatrics, The University of Alabama School of Medicine, CHB 116, 1600 6th Avenue South, Birmingham, AL, USA. sross@peds.uab.edu.
University of Alabama at Birmingham · USUniversity of Alabama · USAlabama Department of Public Health · US

Funding

Viral diversity in congenital cytomegalovirus infectionR01DC012661 · NIDCD · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI ROSS, SHANNON A · 2012 to 2016
$2.3M
NIDCD NIH HHS R01 DC012661NIH HHS R01-DC012661-01
6 · The paper itself

Abstract

backgroundCongenital cytomegalovirus (cCMV) infection is the most common congenital infection and a leading cause of long-term neurological and sensory sequelae, the most common being sensorineural hearing loss (SNHL). Despite extensive research, clinical or laboratory markers to identify CMV infected children with increased risk for disease have not been identified. This study utilizes viral whole-genome next generation-sequencing (NGS) of specimens from congenitally infected infants to explore viral diversity and specific viral variants that may be associated with symptomatic infection and SNHL.

methodsCMV DNA from urine specimens of 30 infants (17 asymptomatic, 13 symptomatic) was target enriched and next generation sequenced resulting in 93% coverage of the CMV genome allowing analysis of viral diversity.

resultsVariant frequency distribution was compared between children with symptomatic and asymptomatic cCMV and those with (n = 13) and without (n = 17) hearing loss. The CMV genes UL48A, UL88, US19 and US22 were found to have an increase in nucleotide diversity in symptomatic children; while UL57, UL20, UL104, US14, UL115, and UL35 had an increase in diversity in children with hearing loss. An analysis of single variant differences between symptomatic and asymptomatic children found UL55 to have the highest number, while the most variants associated with SNHL were in the RL11 gene family. In asymptomatic infants with SNHL, mutations were observed more frequently in UL33 and UL20.

conclusionCMV genomes from infected newborns can be mapped to 93% of the genome at a depth allowing accurate and reproducible analysis of polymorphisms for variant and gene discovery that may be linked to symptomatic and hearing loss outcomes.

Indexed as

ChildCytomegalovirusCytomegalovirus InfectionsDNA, ViralFemaleHearing Loss, SensorineuralHigh-Throughput Nucleotide SequencingHumansInfantInfant, NewbornMaleMutationPhylogenyPrincipal Component AnalysisDNA, ViralCytomegalovirus (CMV)Next generation sequencing (NGS)Sensory neural hearing loss (SNHL)Viral diversity

Identifiers

PMID31822287
PMCPMC6905059
OpenAlexW2994640953

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.