Evidence map›Paper›PMID 31822215›Full record

ArticleStem cells and development2020

Development of Male External Urethral Sphincter and Tissue-Resident Stem/Progenitor Cells in Rats.

Feng Zhou, Amanda B Reed-Maldonado, Yan Tan, Huixing Yuan, Dongyi Peng, Lia Banie, Guifang Wang, Jianquan Hou, Guiting Lin, Tom F Lue

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells and development, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Feng ZhouKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Amanda B Reed-MaldonadoKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Yan TanKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Huixing YuanKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Dongyi PengKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Lia BanieKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Guifang WangKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Jianquan HouDepartment of Urology, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou, China.
Guiting LinKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
Tom F LueKnuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California.
University of California, San Francisco · USTripler Army Medical Center · US

Funding

Therapy for Obesity-associated Stress Urinary IncontinenceR01DK105097 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LUE, TOM F · 2016 to 2019
$2.6M
NIDDK NIH HHS R01 DK105097
6 · The paper itself

Abstract

Stress urinary incontinence (SUI) after prostate surgery is primarily caused by urethral sphincter damage. There are few effective therapeutic approaches for male SUI due to both insufficient study of the structure of the external urethral sphincter (EUS) and incomplete understanding of the resident EUS stem/progenitor cells. The goals of this study were to localize and to determine the distribution of tissue-resident stem/progenitor cells in the male EUS throughout EUS development and to understand the anatomic temporal patterns of the EUS. Newborn Sprague Dawley rats were intraperitoneally injected with the thymidine analogue, 5-ethynyl-2-deoxyuridine (EdU), and the EUS was harvested at five time points (1, 2, 3, 4, and 8 weeks postinjection). The tissue was then processed for EdU staining and immunofluorescence staining for stem cell markers Ki67 and proliferating cell nuclear antigen. We counted the EdU+ label-retaining cells (LRCs) at each time point and colocalized with each stem cell marker, also we isolated and cultured the cells in vitro. The results revealed that the number of EdU+ LRCs in each EUS cross-section decreased over time and that the LRCs were located immediately under the basal membrane of laminin, densely adherent to the muscle fibers. In addition, the thickness of the striated muscle layer developed much faster than the smooth muscle layer during EUS development. By 4 weeks, the structure of the EUS layers was well differentiated. The EUS resident stem/progenitor cells were isolated with MACS

Indexed as

Muscle DevelopmentAdult Stem CellsAnimalsCells, CulturedCell Self RenewalMaleRatsRats, Sprague-DawleyUrethraexternal urethral sphinctermale stress urinary incontinencemicturitionmyogenesisstem/progenitor cells

Identifiers

PMID31822215
PMCPMC6987740
OpenAlexW2996535007

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.