Evidence map›Paper›PMID 31820709›Full record

ArticleThe American journal of tropical medicine and hygiene2020

Oxford Nanopore MinION Sequencing Enables Rapid Whole Genome Assembly of

Ivo Elliott, Elizabeth M Batty, Damien Ming, Matthew T Robinson, Pruksa Nawtaisong, Mariateresa de Cesare, Paul N Newton, Rory Bowden

Abstract read
In one paragraph

Article in The American journal of tropical medicine and hygiene, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Genetic typing of isolates of Rickettsia typhi.PLoS neglected tropical diseases · 2022
    Article
  12. Article
  13. Article
  14. Article
  15. Manifestations and Management of Flea-Borne Rickettsioses.Research and reports in tropical medicine · 2021
    Review
  16. Article
  17. Lysenin Channels as Sensors for Ions and Molecules.Sensors (Basel, Switzerland) · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ivo ElliottNuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.
Elizabeth M BattyMahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Damien MingLao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR.
Matthew T RobinsonNuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.
Pruksa NawtaisongLao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR.
Mariateresa de CesareWellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.
Paul N NewtonNuffield Department of Medicine, Centre for Tropical Medicine and Global Health, University of Oxford, Oxford, United Kingdom.
Rory BowdenWellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.

Funding

Wellcome Trust 090532/Z/09/ZWellcome Trust 105731/Z/14/ZWellcome Trust 203141/Z/16/Z
6 · The paper itself

Abstract

The infrastructure challenges and costs of next-generation sequencing have been largely overcome, for many sequencing applications, by Oxford Nanopore Technologies' portable MinION sequencer. However, the question remains open whether MinION-based bacterial whole genome sequencing is by itself sufficient for the accurate assessment of phylogenetic and epidemiological relationships between isolates and whether such tasks can be undertaken in resource-limited settings. To investigate this question, we sequenced the genome of an isolate of

Indexed as

Genome, BacterialDNA, BacterialHumansNucleic Acid Amplification TechniquesRickettsia typhiDNA, Bacterial

Identifiers

PMID31820709
PMCPMC7008338

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.