Evidence map›Paper›PMID 31820311›Full record

ArticleMolecular biology reports2020

Development of microsatellite markers for the threatened species Coleocephalocereus purpureus (Cactaceae) using next-generation sequencing.

Daphne Amaral Fraga, Anderson Figueiredo de Carvalho, Ricardo Souza Santana, Marlon Câmara Machado, Gustavo Augusto Lacorte

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Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Daphne Amaral FragaMolecular Biology Lab, Department of Science and Languages, Federal Institute of Minas Gerais, Bambuí Campus, Bambuí, Minas Gerais State, Brazil.
Anderson Figueiredo de CarvalhoMolecular Biology Lab, Department of Science and Languages, Federal Institute of Minas Gerais, Bambuí Campus, Bambuí, Minas Gerais State, Brazil.
Ricardo Souza SantanaNativa Serviços Ambientais Ltda, Curvelo, Minas Gerais State, Brazil.ORCID http://orcid.org/0000-0002-1107-3040
Marlon Câmara MachadoMolecular Biology Lab, Department of Science and Languages, Federal Institute of Minas Gerais, Bambuí Campus, Bambuí, Minas Gerais State, Brazil.
Gustavo Augusto LacorteMolecular Biology Lab, Department of Science and Languages, Federal Institute of Minas Gerais, Bambuí Campus, Bambuí, Minas Gerais State, Brazil. gustavo.lacorte@ifmg.edu.br.ORCID http://orcid.org/0000-0002-6866-348X

Funding

FAPEMIG Processo: MPR-01008-16Instituto Federal de Minas Gerais Edital de Pesquisa Aplicada (Edital 104-2016)
6 · The paper itself

Abstract

Ten microsatellite loci were developed and validated for the endangered cactus species Coleocephalocereus purpureus. The markers were obtained from sequences generated by whole genome shotgun sequencing approaches. A testing group of 36 specimens of the main population were genotyped and all described markers presented suitable outcomes to population genetic studies, showing polymorphic status for C. purpureus testing group with clean and reproducible amplification. No evidence for scoring errors, null alleles or linkage disequilibrium was detected. Number of alleles per locus ranged from 3 to 6 and expected heterozygosity ranged from 0.78 to 0.99. These new microsatellite loci are suitable to be used in future diversity and structure population studies of C. purpureus.

Indexed as

Endangered SpeciesHigh-Throughput Nucleotide SequencingBase SequenceCactaceaeGenetic LociMicrosatellite RepeatsCactusEndangered speciesMicrosatellitesPopulation genetics

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.