Evidence map›Paper›PMID 31820078›Full record

ArticleEuropean journal of nutrition2020

Trans-10-hydroxy-2-decenoic acid protects against LPS-induced neuroinflammation through FOXO1-mediated activation of autophagy.

Mengmeng You, Zhuoning Miao, Jing Tian, Fuliang Hu

Abstract read
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In one paragraph

Article in European journal of nutrition, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 38 citations in OpenAlex.

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  12. Role of α-synuclein in microglia: autophagy and phagocytosis balance neuroinflammation in Parkinson's disease.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Mengmeng YouCollege of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Zhuoning MiaoCollege of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Jing TianCollege of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Fuliang HuCollege of Animal Sciences, Zhejiang University, Hangzhou, 310058, China. flhu@zju.edu.cn.ORCID http://orcid.org/0000-0002-2457-7475
Zhejiang University · CN

Funding

Modern Agro-industry Technology Research System from the Ministry of Agriculture of China CARS-45National Natural Science Foundation of China 31872431
6 · The paper itself

Abstract

purposeNeuroinflammation is thought to be associated with the pathogenesis of a series of neurodegenerative diseases. We have previously reported that royal jelly (RJ) has an anti-inflammatory effect on microglial BV-2 cells. However, components contributing to the effect of RJ were largely unexplored. The aim of this study was to assess whether trans-10-hydroxy-2-decenoic acid (10-HDA), the exclusive fatty acid in RJ, can alleviate neuroinflammation and to further explore the underlying mechanisms.

methodsImmunohistochemistry staining, ELISA, qRT-PCR and Western blot were used to assess the effect of 10-HDA on LPS-induced neuroinflammation both in vivo and in vitro. To determine the extent of inflammatory changes after 10-HDA treatment, RNAseq transcriptomic analysis was conducted.

results10-HDA pretreatment significantly reduced the production of pro-inflammatory mediators in LPS-treated C57BL/6J mice and microglial BV-2 cells. 10-HDA inhibited the activation of the TNF-α/NF-κB axis and NLRP3 inflammasome-IL-1β pathway, which may be the anti-neuroinflammatory mechanism of 10-HDA. We also demonstrated that 10-HDA triggered cell autophagy, as evidenced by elevated levels of microtubule-associated protein 1 light chain 3-II (LC3-II) and decreased expression of SQSTM1. More importantly, 10-HDA increased the transcriptional activity of FOXO1 by increasing FOXO1 nuclear localization. Inhibition of FOXO1 and autophagy using chemical inhibitors markedly blunted the effect of 10-HDA on the TNF-α pathway and NLRP3 inflammasome-IL-1β pathway, indicating that 10-HDA alleviates neuroinflammation in BV-2 cells by modulating FOXO1-mediated autophagy.

conclusions10-HDA may be a promising agent for various neuroinflammation-associated diseases.

Indexed as

LipopolysaccharidesSignal TransductionAnimalsAutophagyFatty Acids, MonounsaturatedForkhead Box Protein O1MiceMice, Inbred C57BLMicrogliaNF-kappa B10-hydroxy-2-decenoic acidFatty Acids, MonounsaturatedForkhead Box Protein O1Foxo1 protein, mouseLipopolysaccharidesNF-kappa BAutophagyFOXO1NeuroinflammationNLRP3 inflammasomeTNF-α/NF-κB axisTrans-10-hydroxy-2-decenoic acid

Identifiers

PMID31820078
OpenAlexW2991658842

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.