ArticleApplied microbiology and biotechnology2020
An exploration of smokeless tobacco product nucleic acids: a combined metagenome and metatranscriptome analysis.
Article in Applied microbiology and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Hyperin application modulates leaf bacterial communities and reduces tobacco-specific nitrosamines in air-cured Burley tobacco.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- The role of Bacterial-Fungal interactions in cigar tobacco fermentation: insights from community dynamics and physicochemical correlations.BMC microbiology · 2026Article
- Bacterial community structure associated with smokeless tobacco reference products under different storage conditions and durations.Frontiers in public health · 2025Article
- 16S rDNA-Based Amplicon Analysis Unveiled a Correlation Between the Bacterial Diversity and Antibiotic Resistance Genes of Bacteriome of Commercial Smokeless Tobacco Products.Applied biochemistry and biotechnology · 2024Article
- Analyzing microbial community and volatile compound profiles in the fermentation of cigar tobacco leaves.Applied microbiology and biotechnology · 2024Article
- Shotgun metagenome sequencing of a Sudanese toombak snuff tobacco: genetic attributes of a high tobacco-specific nitrosamine containing smokeless tobacco product.Letters in applied microbiology · 2022Article
- Characteristics of fungal communities and the sources of mold contamination in mildewed tobacco leaves stored under different climatic conditions.Applied microbiology and biotechnology · 2022Article
- Associations between microbial communities and key chemical constituents in U.S. domestic moist snuff.PloS one · 2022Article
- Phyllosphere microbial community of cigar tobacco and its corresponding metabolites.Frontiers in microbiology · 2022Article
- Microorganisms: crucial players of smokeless tobacco for several health attributes.Applied microbiology and biotechnology · 2021Review
- Microbiology of the American Smokeless Tobacco.Applied microbiology and biotechnology · 2021Review
- A roadmap from unknowns to knowns: Advancing our understanding of the microbiomes of commercially available tobacco products.Applied microbiology and biotechnology · 2021Review
- Bacteriome of Moist Smokeless Tobacco Products Consumed in India With Emphasis on the Predictive Functional Potential.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Smokeless tobacco (ST) products are used worldwide and are a major public health concern. In addition to harmful chemicals found in these products, microbes found in ST products are believed to be responsible for generating harmful tobacco-specific nitrosamines (TSNAs), the most abundant carcinogens in ST. These microbes also contribute endotoxins and other pro-inflammatory components. A greater understanding of the microbial constituents in these products is sought in order to potentially link select design aspects or manufacturing processes to avoidable increases in harmful constituents. Previous studies looked primarily at bacterial constituents and had not differentiated between viable vs nonviable organisms, so in this study, we sought to use a dual metatranscriptomic and metagenomic analysis to see if differences exist. Using high-throughput sequencing, we observed that there were differences in taxonomic abundances between the metagenome and metatranscriptome, and in the metatranscriptome, we also observed an abundance of plant virus RNA not previously reported in DNA-only studies. We also found in the product tested, that there were no viable bacteria capable of metabolizing nitrate to nitrite. Therefore, the product tested would not be likely to increase TSNAs during shelf storage. We tested only a single product to date using the strategy presented here, but succeeded in demonstrating the value of using of these methods in tobacco products. These results present novel findings from the first combined metagenome and metatranscriptome of a commercial tobacco product.
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