ArticlePlant biotechnology (Tokyo, Japan)2018
Comparative analysis of CYP716A subfamily enzymes for the heterologous production of C-28 oxidized triterpenoids in transgenic yeast.
Article in Plant biotechnology (Tokyo, Japan), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 31 citations in OpenAlex.
- A modular UDP-glycosyltransferase and cytochrome P450 system for soyasaponin C-22 modification.The Plant journal : for cell and molecular biology · 2026Article
- Integrated ITS2 DNA Barcoding and LC-QTOF/MS Metabolomics for Authentication of Ngueak Pla Mo (Plants (Basel, Switzerland) · 2026Article
- Metabolic and genetic analysis links TRITERPENE SYNTHASE 12 to oleanolic acid biosynthesis in grape berry wax.Journal of experimental botany · 2025Article
- Independent evolution of betulin biosynthesis in Inonotus obliquus.Scientific reports · 2025Article
- A molecular representation system with a common reference frame for analyzing triterpenoid structural diversity.Plant communications · 2025Article
- Integrated transcriptome and metabolome analyses reveal key genes regulating jujuboside biosynthesis inFrontiers in plant science · 2025Article
- Recent trends in the elucidation of complex triterpene biosynthetic pathways in horticultural trees.Horticulture research · 2025Article
- Heterologous production of corosolic acid, a phyto-insulin, in agroinfiltratedPlant biotechnology (Tokyo, Japan) · 2024Article
- OeBAS and CYP716C67 catalyze the biosynthesis of health-beneficial triterpenoids in olive (Olea europaea) fruits.The New phytologist · 2023Article
- Engineering ofMetabolic engineering communications · 2022Article
- Metabolic stimulation-elicited transcriptional responses and biosynthesis of acylated triterpenoids precursors in the medicinal plant Helicteres angustifolia.BMC plant biology · 2022Article
- Identification of key amino acid residues toward improving the catalytic activity and substrate specificity of plant-derived cytochrome P450 monooxygenases CYP716A subfamily enzyme for triterpenoid production inFrontiers in bioengineering and biotechnology · 2022Article
- High-yield bioactive triterpenoid production by heterologous expression inFrontiers in plant science · 2022Article
- Comparative Analysis of NADPH-Cytochrome P450 Reductases From Legumes for Heterologous Production of Triterpenoids in TransgenicFrontiers in plant science · 2021Article
- Boosting the biosynthesis of betulinic acid and related triterpenoids in Yarrowia lipolytica via multimodular metabolic engineering.Microbial cell factories · 2019Article
- Molecular Basis of C-30 Product Regioselectivity of Legume Oxidases Involved in High-Value Triterpenoid Biosynthesis.Frontiers in plant science · 2019Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several enzymes of the CYP716A subfamily have been reported to be involved in triterpenoid biosynthesis. Members of this subfamily oxidize various positions along the triterpenoid backbone and the majority of them catalyze a three-step oxidation at the C-28 position. Interestingly, C-28 oxidation is a common feature in oleanolic acid, ursolic acid, and betulinic acid, which are widely distributed in plants and exhibit important biological activities. In this work, three additional CYP716A enzymes isolated from olive, sugar beet, and coffee, were characterized as multifunctional C-28 oxidases. Semi-quantitative comparisons of in vivo catalytic activity were made against the previously characterized enzymes CYP716A12, CYP716A15, and CYP716A52v2. When heterologously expressed in yeast, the isolated enzymes differed in both catalytic activity and substrate specificity. This study indicates that the screening of enzymes from different plants could be a useful means of identifying enzymes with enhanced catalytic activity and desired substrate specificity. Furthermore, we show that "naturally-evolved" enzymes can be useful in the heterologous production of pharmacologically and industrially important triterpenoids.
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