Evidence map›Paper›PMID 31818904›Full record

ArticlePlant physiology2020

Rapid Single-Step Affinity Purification of HA-Tagged Plant Mitochondria.

Franziska Kuhnert, Anja Stefanski, Nina Overbeck, Leonie Drews, Andreas S Reichert, Kai Stühler, Andreas P M Weber

Open access · greenAbstract read
In one paragraph

Article in Plant physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 41 citations in OpenAlex.

  1. Therapeutic Potential of Mitochondrial Transplantation with Focus on DBD.International journal of molecular sciences · 2026
    Review
  2. Article
  3. Review
  4. Other Techniques.Advances in biochemical engineering/biotechnology · 2026
    Review
  5. Review
  6. In Vivo Epitope Tagging of Plant Mitochondria.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  7. Isolation of Plant Mitochondria Using Affinity Purification.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  8. Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP).Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Plant expression of NifD protein variants resistant to mitochondrial degradation.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Franziska KuhnertInstitute of Plant Biochemistry, Cluster of Excellence on Plant Science (CEPLAS), Heinrich Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany.ORCID 0000-0002-0374-6671
Anja StefanskiMolecular Proteomics Laboratory, Biomedical Research Center, Heinrich Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany.
Nina OverbeckMolecular Proteomics Laboratory, Biomedical Research Center, Heinrich Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany.
Leonie DrewsInstitute of Biochemistry and Molecular Biology I, Medical Faculty, Heinrich-Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany.
Andreas S ReichertInstitute of Biochemistry and Molecular Biology I, Medical Faculty, Heinrich-Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany.
Kai StühlerMolecular Proteomics Laboratory, Biomedical Research Center, Heinrich Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany.
Andreas P M WeberInstitute of Plant Biochemistry, Cluster of Excellence on Plant Science (CEPLAS), Heinrich Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany aweber@hhu.de.ORCID 0000-0003-0970-4672
Heinrich Heine University Düsseldorf · DECluster of Excellence on Plant Sciences · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Photosynthesis in plant cells would not be possible without the supportive role of mitochondria. However, isolating mitochondria from plant cells for physiological and biochemical analyses is a lengthy and tedious process. Established isolation protocols require multiple centrifugation steps and substantial amounts of starting material. To overcome these limitations, we tagged mitochondria in Arabidopsis (

Indexed as

MitochondriaArabidopsisArabidopsis ProteinsChromatography, AffinityDNA-Binding ProteinsGlutamineHemagglutinin Glycoproteins, Influenza VirusMembrane Transport ProteinsMicroscopy, ConfocalMitochondrial Membrane Transport ProteinsPeptide FragmentsPlants, Genetically ModifiedProteomeProteomicsSeedlingsTranscription Factorsa bout de souffle protein, ArabidopsisArabidopsis ProteinsCONSTANS protein, ArabidopsisDNA-Binding ProteinsGlutamineHemagglutinin Glycoproteins, Influenza VirusMembrane Transport ProteinsMitochondrial Membrane Transport ProteinsPeptide FragmentsProteomeTranscription Factorstyrosyl-prolyl-tyrosyl-aspartyl-valyl-prolyl-aspartyl-tyrosyl-alanine

Identifiers

PMID31818904
PMCPMC6997695
OpenAlexW2996724171

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.