Evidence map›Paper›PMID 31818691›Full record

ArticleMolecular therapy : the journal of the American Society of Gene Therapy2020

Urinary Extracellular Vesicles Carrying Klotho Improve the Recovery of Renal Function in an Acute Tubular Injury Model.

Cristina Grange, Elli Papadimitriou, Veronica Dimuccio, Cecilia Pastorino, Jordi Molina, Ryan O'Kelly, Laura J Niedernhofer, Paul D Robbins, Giovanni Camussi, Benedetta Bussolati

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 68 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed, 1 pooled it
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed, 1 synthesis or guideline pooled it, 104 citations in OpenAlex.

  1. Guideline
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  5. Article
  6. Integrative Approaches to Treating Cellular Senescence in Kidney Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  7. Article
  8. Review
  9. The role of extracellular vesicles in kidney disease progression.Kidney research and clinical practice · 2026
    Article
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  11. Article
  12. Article
  13. Article
  14. Review
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  17. Article
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8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Cristina GrangeDepartment of Medical Sciences, University of Turin, Turin, Italy.
Elli PapadimitriouDepartment of Molecular Biotechnology and Health Sciences, University of Turin, Turin, Italy.
Veronica DimuccioDepartment of Molecular Biotechnology and Health Sciences, University of Turin, Turin, Italy.
Cecilia PastorinoDepartment of Molecular Biotechnology and Health Sciences, University of Turin, Turin, Italy.
Jordi MolinaDepartment of Molecular Biotechnology and Health Sciences, University of Turin, Turin, Italy.
Ryan O'KellyInstitute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN, USA.
Laura J NiedernhoferInstitute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN, USA.
Paul D RobbinsInstitute on the Biology of Aging and Metabolism, Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota Medical School, Minneapolis, MN, USA.
Giovanni CamussiDepartment of Medical Sciences, University of Turin, Turin, Italy.
Benedetta BussolatiDepartment of Molecular Biotechnology and Health Sciences, University of Turin, Turin, Italy. Electronic address: benedetta.bussolati@unito.it.
University of Turin · ITUniversity of Minnesota · US

Funding

PROTEOMICSP01AG043376 · NIA · UNIVERSITY OF MINNESOTA · PI ROBBINS, PAUL D. · 2013 to 2019
$11.2M
Extracellular vesicles as cell non-autonomous geronic factorsR56AG059675 · NIA · UNIVERSITY OF MINNESOTA · PI ROBBINS, PAUL D. · 2018 to 2018
$385k
NIA NIH HHS P01 AG043376NIA NIH HHS R56 AG059675
6 · The paper itself

Abstract

Acute kidney injury, defined by a rapid deterioration of renal function, is a common complication in hospitalized patients. Among the recent therapeutic options, the use of extracellular vesicles (EVs) is considered a promising strategy. Here we propose a possible therapeutic use of renal-derived EVs isolated from normal urine (urine-derived EVs [uEVs]) in a murine model of acute injury generated by glycerol injection. uEVs accelerated renal recovery, stimulating tubular cell proliferation, reducing the expression of inflammatory and injury markers, and restoring endogenous Klotho loss. When intravenously injected, labeled uEVs localized within injured kidneys and transferred their microRNA cargo. Moreover, uEVs contained the reno-protective Klotho molecule. Murine uEVs derived from Klotho null mice lost the reno-protective effect observed using murine EVs from wild-type mice. This was regained when Klotho-negative murine uEVs were reconstituted with recombinant Klotho. Similarly, ineffective fibroblast EVs acquired reno-protection when engineered with human recombinant Klotho. Our results reveal a novel potential use of uEVs as a new therapeutic strategy for acute kidney injury, highlighting the presence and role of the reno-protective factor Klotho.

Indexed as

Acute Kidney InjuryAnimalsBiomarkersCytokinesExtracellular VesiclesGlucuronidaseImmunohistochemistryInflammation MediatorsKidney Function TestsKidney TubulesKlotho ProteinsMiceBiomarkersCytokinesGlucuronidaseInflammation MediatorsKlotho ProteinsAKIdamageengineeringinjury markersKlothomesenchymal stem cellsmicroRNAsrenal regenerationurinary exosomesvesicles

Identifiers

PMID31818691
PMCPMC7000999
OpenAlexW2987023410

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.