Evidence map›Paper›PMID 31816179›Full record

ArticleCurrent protocols in microbiology2019

Laboratory Cultivation and Storage of Shigella.

Shelley M Payne

Abstract read
In one paragraph

Article in Current protocols in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Optimization ofJournal of environmental engineering (New York, N.Y.) · 2025
    Article
  5. Article
  6. Epithelial Cell Infection Analyses with Shigella.Journal of visualized experiments : JoVE · 2024
    Article
  7. Gut microbes · 2023
    Article
  8. GeneratingFrontiers in oncology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Shelley M PayneDepartment of Molecular Biosciences, University of Texas at Austin, Austin, Texas.

Funding

ROLE OF CARBON METABOLISM AND VIRULENCE OF SHIGELLA FLEXNERIR37AI016935 · NIAID · UNIVERSITY OF TEXAS AUSTIN · PI Shelley M. Payne · 2003 to 2026
$6.0M
Role of carbon metabolism in virulence of Shigella flexneriR01AI016935 · NIAID · UNIVERSITY OF TEXAS AUSTIN · PI PAYNE, SHELLEY M. · 1985 to 2017
$2.6M
NIAID NIH HHS R01 AI016935NIAID NIH HHS R37 AI016935
6 · The paper itself

Abstract

Shigella species, which are closely related to Escherichia coli, can easily be maintained and stored in the laboratory. This article includes protocols for preparation of routine growth conditions and media, for storage of the bacteria, and for monitoring of the presence of the virulence plasmid. © 2019 by John Wiley & Sons, Inc. Basic Protocol 1: Growth of S. flexneri from frozen stocks or agar stabs Basic Protocol 2: Growth of S. flexneri in rich liquid medium Alternate Protocol 1: Growth of S. flexneri in rich defined medium Alternate Protocol 2: Growth of S. flexneri in minimal medium Basic Protocol 3: Storage of S. flexneri in frozen stocks Alternate Protocol 3: Storage of S. flexneri in agar stabs.

Indexed as

Bacteriological TechniquesCulture MediaPlasmidsPreservation, BiologicalShigellaCulture Mediabacterial cultivationShigellaShigella flexneri

Identifiers

PMID31816179
PMCPMC7274091

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.