Evidence map›Paper›PMID 31813827›Full record

ArticleStem cell reports2019

Precision Health Resource of Control iPSC Lines for Versatile Multilineage Differentiation.

Matthew R Hildebrandt, Miriam S Reuter, Wei Wei, Naeimeh Tayebi, Jiajie Liu, Sazia Sharmin, Jaap Mulder, L Stephen Lesperance, Patrick M Brauer, Rebecca S F Mok and 18 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed.

  1. Article
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  9. ARP2/3 complex mediates the neuropathology of PTEN-deficient human neural cells downstream of mTORC1 and mTORC2 hyperactivation.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  10. Article
  11. USH2A-Mutated Human Retinal Organoids Model Rod-Cone Dystrophy.Investigative ophthalmology & visual science · 2025
    Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Review
  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors.

Matthew R HildebrandtDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Miriam S ReuterGenetics and Genome Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; The Centre for Applied Genomics, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Wei WeiDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Naeimeh TayebiGenetics and Genome Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Jiajie LiuDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Sazia SharminDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Jaap MulderDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
L Stephen LesperanceNeurosciences and Mental Health, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Patrick M BrauerDepartment of Immunology, University of Toronto, Sunnybrook Research Institute, Toronto, ON M4N 3M5, Canada.
Rebecca S F MokDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
Caroline KinnearGenetics and Genome Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Alina PieknaDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Asli RommDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Jennifer HoweGenetics and Genome Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; The Centre for Applied Genomics, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Peter PasceriDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Guoliang MengDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Matthew RozyckiDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Deivid C RodriguesDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
Elisa C MartinezDepartment of Immunology, University of Toronto, Sunnybrook Research Institute, Toronto, ON M4N 3M5, Canada.
Michael J SzegoDalla Lana School of Public Health, University of Toronto, Toronto, ON M5T 3M7, Canada; Department of Family and Community Medicine, University of Toronto, Toronto, ON M5C 2T2, Canada; The Joint Centre for Bioethics, University of Toronto, Toronto, ON, Canada; Unity Health Toronto, Toronto, ON M5T 3M6, Canada.
Juan C Zúñiga-PflückerDepartment of Immunology, University of Toronto, Sunnybrook Research Institute, Toronto, ON M4N 3M5, Canada.
Michele K AndersonDepartment of Immunology, University of Toronto, Sunnybrook Research Institute, Toronto, ON M4N 3M5, Canada.
Steven A PrescottNeurosciences and Mental Health, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada; Department of Physiology, University of Toronto, Toronto, ON M5S 1A8, Canada.
Norman D RosenblumDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Department of Pediatrics, University of Toronto, Toronto, ON M5G 1X8, Canada.
Binita M KamathDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Department of Pediatrics, University of Toronto, Toronto, ON M5G 1X8, Canada.
Seema MitalGenetics and Genome Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Department of Pediatrics, University of Toronto, Toronto, ON M5G 1X8, Canada.
Stephen W SchererGenetics and Genome Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; The Centre for Applied Genomics, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada; McLaughlin Centre, University of Toronto, Toronto, ON M5G 0A4, Canada. Electronic address: stephen.scherer@sickkids.ca.
James EllisDevelopmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada. Electronic address: jellis@sickkids.ca.

Funding

The role of TCR, ligand, and signal strength in human γδ T cell developmentP01AI102853 · NIAID · RESEARCH INST OF FOX CHASE CAN CTR · PI DAVID L. WIEST · 2014 to 2026
$26.3M
CIHR SOP-155609CIHR THC-135232NIAID NIH HHS P01 AI102853
6 · The paper itself

Abstract

Induced pluripotent stem cells (iPSC) derived from healthy individuals are important controls for disease-modeling studies. Here we apply precision health to create a high-quality resource of control iPSCs. Footprint-free lines were reprogrammed from four volunteers of the Personal Genome Project Canada (PGPC). Multilineage-directed differentiation efficiently produced functional cortical neurons, cardiomyocytes and hepatocytes. Pilot users demonstrated versatility by generating kidney organoids, T lymphocytes, and sensory neurons. A frameshift knockout was introduced into MYBPC3 and these cardiomyocytes exhibited the expected hypertrophic phenotype. Whole-genome sequencing-based annotation of PGPC lines revealed on average 20 coding variants. Importantly, nearly all annotated PGPC and HipSci lines harbored at least one pre-existing or acquired variant with cardiac, neurological, or other disease associations. Overall, PGPC lines were efficiently differentiated by multiple users into cells from six tissues for disease modeling, and variant-preferred healthy control lines were identified for specific disease settings.

Indexed as

Cell DifferentiationCell LineageCell Self RenewalCell SeparationCRISPR-Cas SystemsEctodermGene EditingHumansInduced Pluripotent Stem CellsMyocytes, CardiacNeuronsOrganoidsPhenotypeT-LymphocytesWhole Genome Sequencingcellular phenotypingcontrol iPSCsdisease modelinggene editingPersonal Genome Project Canadawhole-genome sequencing

Identifiers

PMID31813827
PMCPMC6915802

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.