ArticleClinical and experimental reproductive medicine2019
Growth differentiation factor 9 and cumulus cell supplementation in in vitro maturation culture media enhances the viability of human blastocysts.
Article in Clinical and experimental reproductive medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Cross-Species Insights intoAntioxidants (Basel, Switzerland) · 2025Article
- Identification of Proteins and MicroRNAs with Prognostic Value for Assisted Reproduction Technology Outcomes in Follicular Fluid of Women with Endometriosis: A Pilot Study.International journal of molecular sciences · 2025Article
- Rescue in vitro maturation of germinal vesicle oocytes after ovarian stimulation: the importance of the culture media.Human reproduction (Oxford, England) · 2025Article
- Current status and hotspots of in vitro oocyte maturation: a bibliometric study of the past two decades.Journal of assisted reproduction and genetics · 2025Review
- Pro-cumulin addition in a biphasic in vitro oocyte maturation system modulates human oocyte and cumulus cell transcriptomes.Molecular human reproduction · 2025Article
- The Roles of GDF-9, BMP-15, BMP-4 and EMMPRIN in Folliculogenesis and In Vitro Fertilization.Journal of clinical medicine · 2024Review
- Prospects for fertility preservation: the ovarian organ function reconstruction techniques for oogenesis, growth and maturationFrontiers in physiology · 2023Review
- Fertility preservation strategies for cancerous women: An updated review.Turkish journal of obstetrics and gynecology · 2022Article
- High efficiency of homemade culture medium supplemented with GDF9-β in human oocytes for rescue in vitro maturation.Clinical and experimental reproductive medicine · 2022Article
- Article
- Factors Influencing the In Vitro Maturation (IVM) of Human Oocyte.Biomedicines · 2021Review
- Human FollicleFrontiers in endocrinology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveIn vitro maturation (IVM) of immature oocytes can be useful for some infertile patients. In IVM programs, the rates of embryo formation and pregnancy are low. Therefore, it is essential to recognize the main factors involved in regulating oocyte maturation in vitro. The purpose of this study was to investigate the effects of growth differentiation factor 9 (GDF9) and cumulus cell (CC) supplementation in IVM medium on the rates of embryo formation and viability of human blastocysts.
methodsA total of 80 germinal vesicle oocytes from stimulated cycles underwent an IVM program. The oocytes were divided into four groups, where group I consisted of IVM media only and served as the control, group II consisted of IVM+CCs, group III consisted of IVM+GDF9 (200 ng/mL), and group IV consisted of IVM+CCs+GDF9 (200 ng/mL). Intracytoplasmic sperm injection was performed on the IVM oocytes, and the cleavage embryos that were generated were vitrified. Following thawing, the embryos were cultured for 3 additional days, and the viability rates of the developed blastocysts were determined.
resultsThe maturation rate of the oocytes did not differ significantly across the four groups. The fertilization rate in group II was significantly higher than that in the control group (76.5% vs. 46.2%). Embryo formation was significantly more frequent in all experimental groups than in the control group, while blastocyst formation did not show significant differences in the three experimental groups compared to the control. The mean viability rates in groups II, III, and IV were 58.16%, 55.91%, and 55.95%, respectively, versus 37.78% in the control group (p<0.05).
conclusionSupplementation of IVM culture media with GDF9 and CCs enhanced the fertilization, embryo formation, and viability rates of blastocysts generated from vitrified cleavage embryos.
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