Evidence map›Paper›PMID 31813129›Full record

ArticleMolecular biology reports2020

In vivo flow cytometry combined with intravital microscopy to monitor kinetics of transplanted bone marrow mononuclear cells in peripheral blood and bone marrow.

Fen Wang, Dan Wei, Yuanzhen Suo, Xi Zhu, Yan Yuan, Wenyuan Gao, Hua Jiang, Xunbin Wei, Tong Chen

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Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 21 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 1 country.

Fen WangDepartment of Hematology, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Dan WeiMed-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Yuanzhen SuoMed-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Xi ZhuMed-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Yan YuanDepartment of Hematology, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Wenyuan GaoDepartment of Pharmacy, Binzhou Medical University, Yantai, 264003, Shandong, China.
Hua JiangObstetrics & Gynecology Hospital, Fudan University, Shanghai, 200011, China. jianghua@fudan.edu.cn.
Xunbin WeiMed-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China. xwei01@sjtu.edu.cn.
Tong ChenDepartment of Hematology, Huashan Hospital, Fudan University, Shanghai, 200040, China. chentong@fudan.edu.cn.
Shanghai Jiao Tong University · CNHuashan Hospital · CNBeihang University · CNBinzhou Medical University · CNFudan University · CNObstetrics and Gynecology Hospital of Fudan University · CN

Funding

National Natural Science Foundation of China 81561138002National Natural Science Foundation of China 91542109National Science Fund for Distinguished Young Scholars 61425006Program of Shanghai Subject Chief Scientist 16XD1400600
6 · The paper itself

Abstract

Bone marrow mononuclear cells (BM-MNCs) transplantation has evolved as a promising experimental treatment in various regenerative therapy fields, especially in clinical hematopoietic stem cells transplantation (HSCT). In vitro methods have mainly been used to study the pre-clinical kinetics of BM-MNCs in mice after transplantation. And it is difficult to monitor the dynamic homing of BM-MNCs in living mice. The present study obtained the kinetics of transplanted BM-MNCs in the peripheral blood (PB) and the dynamic homing of BM-MNCs in the BM in living mice by a combination of in vivo flow cytometry (IVFC) and calvarium intravital microscopy. We found out that BM-MNCs were cleared rapidly from the PB and mainly localized to various hematopoietic tissues after transplantation. The number of BM-MNCs in the PB decreased over time accompanied by an increase in the BM indeed after transplantation. In addition, a lower number of BM-MNCs were found home to calvaria than long bone, probably indicating long bone marrow might also be an important hematopoietic organ. Clinical studies will benefit from non-invasive measurements to monitor the dynamic homing of transplanted cells. Our pre-clinical kinetics of BM-MNCs in living mice will have important clinical guiding significance in HSCT and other regenerative therapy fields.

Indexed as

Bone Marrow TransplantationCell MovementAnimalsBlood CellsBone MarrowBone Marrow CellsCell TrackingChemotaxis, LeukocyteFlow CytometryGreen Fluorescent ProteinsHindlimbIntravital MicroscopyKineticsMiceMice, Inbred BALB CMice, Inbred C57BLenhanced green fluorescent proteinGreen Fluorescent ProteinsBone marrow mononuclear cellsHematopoietic stem cell transplantationHomingIn vivoStem cell

Identifiers

PMID31813129
OpenAlexW2992862477

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.