Evidence map›Paper›PMID 31812534›Full record

ArticleAnalytical biochemistry2020

Integrated approach for data acquisition, visualization and processing of analog polarographic systems for bioenergetics studies.

Potter L, Krusienski D, Kennedy J, Hoppel Cl, Lai N

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 88% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Potter LDepartment of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA, USA; Biomedical Engineering Institute, Old Dominion University, Norfolk, VA, USA.
Krusienski DDepartment of Biomedical Engineering Virginia Commonwealth University, Richmond, VA, USA.
Kennedy JDepartment of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA, USA.
Hoppel ClCenter for Mitochondrial Disease, School of Medicine, Case Western Reserve University, USA; Department of Pharmacology and School of Medicine, Case Western Reserve University, USA; Department of Medicine, School of Medicine, Case Western Reserve University, USA.
Lai NDepartment of Electrical and Computer Engineering, Old Dominion University, Norfolk, VA, USA; Biomedical Engineering Institute, Old Dominion University, Norfolk, VA, USA; Department of Biomedical Engineering, School of Medicine, Case Western Reserve University, USA; Department of Mechanical, Chemical and Materials Engineering, University of Cagliari, Italy. Electronic address: nlai@odu.edu.
Case Western Reserve University · USOld Dominion University · USVirginia Commonwealth University · US

Funding

Translational Research in Cystic FibrosisP30DK027651 · NIDDK · CASE WESTERN RESERVE UNIVERSITY · PI DRUMM, MITCHELL L · 1986 to 2014
$15.6M
Systems Biology Investigation of Muscle Exercise Metabolism in DiabetesK25AR057206 · NIAMS · OLD DOMINION UNIVERSITY · PI LAI, NICOLA · 2011 to 2015
$593k
NIAMS NIH HHS K25 AR057206NIDDK NIH HHS P30 DK027651
6 · The paper itself

Abstract

Bioenergetic function is characterized with assays obtained by polarographic systems. Analog systems without data acquisition, visualization, and processing tools are used but require cumbersome operations to derive respiration rate and ADP to oxygen stoichiometry of oxidative phosphorylation (ADP/O ratio). The analog signal of a polarograhic system (YSI-5300) was digitized and a graphical user interface (GUI) was developed in MATLAB to integrate visualization, recording, calibration and processing of bioenergetic data. With the GUI, the signal is continuously visualized during the experiment and respiratory rates and ADP/O ratios can be determined. The integrated system was tested to evaluate bioenergetic function of subpopulations of mitochondria isolated from rat skeletal muscle (n = 10). Signal processing was applied to denoise data recorded at the sampling rate of 1000Hz, and maximize data decimation for computational applications. The error in calculating the bioenergetic outputs using decimated data is negligible when data are denoised. The estimate of respiration rate, ADP/O ratio and RCR obtained with denoised data at sampling rate as low as 5Hz was similar to that obtained with raw data recorded at sampling rate of 1000Hz. In summary, the integrated tools of the GUI overcome the limitations of data processing, accuracy, and utilization of analog polarographic systems.

Indexed as

SoftwareAdenosine DiphosphateAnimalsComputational BiologyMaleMitochondriaOxidative PhosphorylationOxygenRatsRats, WistarAdenosine DiphosphateOxygenADP/O ratioComputational methodsKineticsMitochondriaRespiration rateSoftware

Identifiers

PMID31812534
PMCPMC6943940
OpenAlexW2992427230

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.