ReviewCurrent microbiology2020
Key Factors for Removing Bias in Viability PCR-Based Methods: A Review.
Review in Current microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?FEMS microbiology reviews · 2023Pooled it
- Simple endpoint PCR assay using portable devices for Escherichia coli quantification in water samples.Environmental monitoring and assessment · 2026Article
- Specific monitoring of viable plant growth-promoting Bacillus velezensis PH-023 in the rhizosphere of tomato.BMC microbiology · 2026Article
- PCR-BasedMicroorganisms · 2025Article
- PMA-qPCR to quantify viable cells in multispecies oral biofilm after disinfectant treatments.Biofilm · 2025Article
- Variations in bacterial profiles associated with semen collection timing and bull breed, analyzed using 16S rRNA sequencing and MALDI-TOF MS.Frontiers in veterinary science · 2025Article
- Optimization of the viability PCR for accurate detection of Staphylococcus aureus in food samples.PloS one · 2025Article
- Revealing the Viable Microbial Community of Biofilm in a Sewage Treatment System Using Propidium Monoazide Combined with Real-Time PCR and Metagenomics.Microorganisms · 2024Article
- Establishment of a Protocol for Viability qPCR in Dental Hard Tissues.Microorganisms · 2024Article
- Quantification and Potential Viability of Human Noroviruses in Final Effluent from Wastewater Treatment Works in Pretoria, South Africa.Food and environmental virology · 2024Article
- Characterization of viable but nonculturable state ofRoyal Society open science · 2024Article
- Improved Canker Processing and Viability Droplet Digital PCR Allow Detection ofMicroorganisms · 2024Article
- Airborne SARS-CoV-2 is more frequently detected in environments related to children and elderly but likely non-infectious, Norway, 2022.Virology journal · 2023Article
- Utilizing the state of environmental DNA (eDNA) to incorporate time-scale information into eDNA analysis.Proceedings. Biological sciences · 2023Review
- Colonization and population dynamics of total, viable, and culturable cells of two biological control strains applied to apricot, peach, and grapevine crops.Frontiers in microbiology · 2023Article
- An Improved Real-Time Viability PCR Assay to DetectInternational journal of molecular sciences · 2022Article
- New immunomagnetic separation method to analyze risk factors for Legionella colonization in health care centres.Journal of exposure science & environmental epidemiology · 2022Article
- A critical analysis of research methods and experimental models to study irrigants and irrigation systems.International endodontic journal · 2022Review
- Quantification of Bacterial DNA from Infected Human Root Canals Using qPCR and DAPI after Disinfection with Established and Novel Irrigation Protocols.Materials (Basel, Switzerland) · 2022Article
- An Assay Combining Droplet Digital PCR With Propidium Monoazide Treatment for the Accurate Detection of Live Cells ofFrontiers in microbiology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the last decade, the viability PCR approach has been investigated intensively with the goal of detecting solely the DNA derived from intact cells by removing the bias resulting from dead or damaged cell artifacts. However, since the first published paper in 2003 and despite the various continuous improvements, the stubborn reality is that in most cases the goal has not been achieved. Complete neutralization of nucleic acids derived from dead microorganisms was not possible, at least without affecting live cells. However, recently published works in this field show how neutralization rates can be improved. In this communication, we review recent improvements that enable effective vPCR procedures.
Indexed as
Identifiers
31811375What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.