Evidence map›Paper›PMID 31811247›Full record

ArticleScientific reports2019

Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies.

Muhammad Ali, Firoz Kabir, Jason J Thomson, Yinghong Ma, Caihong Qiu, Michael Delannoy, Shahid Y Khan, S Amer Riazuddin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Muhammad AliThe Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Firoz KabirThe Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
Jason J ThomsonYale Stem Cell Center, Yale University School of Medicine, New Haven, CT 06520, USA.
Yinghong MaYale Stem Cell Center, Yale University School of Medicine, New Haven, CT 06520, USA.
Caihong QiuYale Stem Cell Center, Yale University School of Medicine, New Haven, CT 06520, USA.
Michael DelannoyDepartment of Cell Biology and Imaging Facility, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Shahid Y KhanThe Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.
S Amer RiazuddinThe Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. riazuddin@jhmi.edu.

Funding

Identification of Lens Function Through Analysis of Human CataractsR01EY022714 · NEI · JOHNS HOPKINS UNIVERSITY · PI RIAZUDDIN, AMER · 2014 to 2018
$2.0M
NEI NIH HHS R01 EY022714U.S. Department of Health & Human Services | NIH | National Eye Institute (NEI) R01EY022714
6 · The paper itself

Abstract

The ocular lens serves as an excellent system to investigate the intricate details of development and differentiation. Generation of lentoid bodies or lens-like structures using pluripotent stem cells is important for understanding the processes critical for lens morphogenesis and the mechanism of cataractogenesis. We previously reported the generation of peripheral blood mononuclear cell (PBMC)-originated, induced pluripotent stem cells (iPSCs). Here, we report generation of lentoid bodies from human embryonic stem cells (hESCs) and (PBMC)-originated, iPSCs employing the "fried egg" method with brief modifications. The ultrastructure analysis of hESC- and iPSC-derived lentoid bodies identified closely packed lens epithelial- and differentiating fiber-like cells. In addition, we performed RNA sequencing (RNA-Seq) based transcriptome profiling of hESC- and iPSC-derived lentoid bodies at differentiation day 25. Next-generation RNA sequencing (RNA-Seq) of hESC- and iPSC-derived lentoid bodies detected expression (≥0.659 RPKM) of 13,975 and 14,003 genes, respectively. Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies revealed 13,563 (>96%) genes common in both datasets. Among the genes common in both transcriptome datasets, 12,856 (~95%) exhibited a quantitatively similar expression profile. Next, we compared the mouse lens epithelial and fiber cell transcriptomes with hESC- and iPSC-derived lentoid bodies transcriptomes and identified > 96% overlap with lentoid body transcriptomes. In conclusion, we report first-time comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies at differentiation day 25.

Indexed as

Gene Expression Regulation, DevelopmentalAgedCell DifferentiationCell LineCellular ReprogrammingHuman Embryonic Stem CellsHumansInduced Pluripotent Stem CellsLens, CrystallineLeukocytes, MononuclearMalePrimary Cell CultureRNA-SeqTranscriptome

Identifiers

PMID31811247
PMCPMC6898283

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.