Evidence map›Paper›PMID 31810440›Full record

ArticleMolecular medicine (Cambridge, Mass.)2019

Adverse transverse-tubule remodeling in a rat model of heart failure is attenuated with low-dose triiodothyronine treatment.

Shimin An, Nimra Gilani, Yuan Huang, Adam Muncan, Youhua Zhang, Yi-Da Tang, A Martin Gerdes, Kaie Ojamaa

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine (Cambridge, Mass.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Cardiovascular Findings and Effects of Caffeine on Experimental Hypothyroidism.Endocrine, metabolic & immune disorders drug targets · 2025
    Article
  6. Article
  7. Nanoscale organization of cardiac calcium channels is dependent on thyroid hormone status.American journal of physiology. Heart and circulatory physiology · 2024
    Article
  8. Article
  9. Triiodothyronine maintains cardiac transverse-tubule structure and function.Journal of molecular and cellular cardiology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Shimin AnDepartment of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, PO Box 8000, Northern Blvd., Old Westbury, New York, 11568, USA.
Nimra GilaniDepartment of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, PO Box 8000, Northern Blvd., Old Westbury, New York, 11568, USA.
Yuan HuangDepartment of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, PO Box 8000, Northern Blvd., Old Westbury, New York, 11568, USA.
Adam MuncanDepartment of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, PO Box 8000, Northern Blvd., Old Westbury, New York, 11568, USA.
Youhua ZhangDepartment of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, PO Box 8000, Northern Blvd., Old Westbury, New York, 11568, USA.
Yi-Da TangDepartment of Cardiology, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Beilishi Road, Beijing, 100037, China.
A Martin GerdesDepartment of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, PO Box 8000, Northern Blvd., Old Westbury, New York, 11568, USA.
Kaie OjamaaDepartment of Biomedical Sciences, New York Institute of Technology College of Osteopathic Medicine, PO Box 8000, Northern Blvd., Old Westbury, New York, 11568, USA. kojamaa@nyit.edu.ORCID 0000-0002-0130-7456
New York Institute of Technology · USChinese Academy of Medical Sciences & Peking Union Medical College · CN

Funding

Low Thyroid function and myocardial infarctionR01HL103671 · NHLBI · NEW YORK INST OF TECHNOLOGY · PI GERDES, ANTHONY MARTIN · 2011 to 2015
$1.8M
NHLBI NIH HHS R01 HL103671
6 · The paper itself

Abstract

Pre-clinical animal studies have shown that triiodothyronine (T3) replacement therapy improves cardiac contractile function after myocardial infarction (MI). We hypothesized that T3 treatment could prevent adverse post-infarction cardiomyocyte remodeling by maintaining transverse-tubule (TT) structures, thus improving calcium dynamics and contractility.

methodsMyocardial infarction (MI) or sham surgeries were performed on female Sprague-Dawley rats (aged 12 wks), followed by treatment with T3 (5μg/kg/d) or vehicle in drinking water for 16 wks (n = 10-11/group). After in vivo echocardiographic and hemodynamic analyses, left ventricular myocytes were isolated by collagenase digestion and simultaneous calcium and contractile transients in single cardiomyocytes were recorded using IonOptix imaging. Live cardiomyocytes were stained with AlexaFluor-488 conjugated wheat germ agglutinin (WGA-488) or di-8-ANEPPS, and multiple z-stack images per cell were captured by confocal microscopy for analysis of TT organization. RTqPCR and immunoblot approaches determined expression of TT proteins.

resultsEchocardiography and in vivo hemodynamic measurements showed significant improvements in systolic and diastolic function in T3- vs vehicle-treated MI rats. Isolated cardiomyocyte analysis showed significant dysfunction in measurements of myocyte relengthening in MI hearts, and improvements with T3 treatment: max relengthening velocity (Vmax, um/s), 2.984 ± 1.410 vs 1.593 ± 0.325, p < 0.05 and time to Vmax (sec), 0.233 ± 0.037 vs 0.314 ± 0.019, p < 0.001; MI + T3 vs MI + Veh, respectively. Time to peak contraction was shortened by T3 treatment (0.161 ± 0.021 vs 0.197 ± 0.011 s., p < 0.01; MI + T3 vs MI + Veh, respectively). Analysis of TT periodicity of WGA- or ANEPPS-stained cardiomyocytes indicated significant TT disorganization in MI myocytes and improvement with T3 treatment (transverse-oriented tubules (TE%): 9.07 ± 0.39 sham, 6.94 ± 0.67 MI + Veh and 8.99 ± 0.38 MI + T3; sham vs MI + Veh, p < 0.001; MI + Veh vs MI + T3, p < 0.01). Quantitative RT-PCR showed that reduced expression of BIN1 (Bridging integrator-1), Jph2 (junctophilin-2), RyR2 (ryanodine receptor) and Ca

Indexed as

Myocytes, CardiacAnimalsCalciumCells, CulturedFemaleHeart FailureRatsRats, Sprague-DawleySarcolemmaTriiodothyronineVentricular RemodelingCalciumTriiodothyronineCalcium transientsContractilityHeart failureTriiodothyronineT-tubules

Identifiers

PMID31810440
PMCPMC6898920
OpenAlexW2993129015

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.